肠道菌群对石榴皮鞣质的分解代谢作用研究

Study of metabolism of tannins from Granati Pericarpium in intestinal flora

目的考察肠道菌群对石榴皮鞣质的分解代谢作用,阐明石榴皮鞣质在肠道的代谢途径。方法将石榴皮鞣质与大鼠肠道菌液共同孵育4、6、8、12、24、48 h后,检测不同时间点溶液中的代谢成分。结果通过LC-MS/MS从石榴皮鞣质肠道菌群孵育液中共鉴别出了10个代谢产物,分别为尿石素B、没食子酸、焦性没食子酸、3-O-甲基没食子酸、尿石素A、甲基化尿石素A、尿石素C、尿石素-M6、葡萄糖醛酸化尿石素A、葡萄糖醛酸化尿石素C。结论石榴皮鞣质在肠道菌群中主要通过代谢酶的水解、还原等作用将其代谢成具有生理活性的小分子化合物,这些化合物有可能是石榴皮鞣质在机体内发挥药理活性的物质基础。

Objective To investigate the metabolism effect of tannins from Granati Pericarpium in intestinal flora for elucidating the metabolic pathway of tannins from Granati Pericarpium in the intestinal flora. Methods Pomegranate tannins were incubated with rats intestinal flora for 4, 6, 8, 12, 24, and 48 h. The content was then detected at different times. Results A total of 10 metabolites were detected in the rats intestinal flora by LC-MS, namely urolithin B, gallic acid, pyrogallic acid, 3-O-methylgallic acid, urolithin A, methyl-urolithin A, urolithin C, urolithin-M6, urolithin A-glucuronidation, urolithin C-glucuronidation. Conclusion Tannins from Granati Pericarpium in intestinal flora could generate physiological activity of small molecule compounds through hydrolysis of metabolic enzymes, and these compounds may be the material basis of tannins from Granati Pericarpium and play the role of pharmacological activities in the body.