小立碗藓萜类物质的合成酶基因分析及其UPLC-QTOF检测

Analysis of Terpenoid Synthase Genes and Detection by UPLC-QTOF Detection in Physcomitrella patens

根据基因组信息和KEGG数据库分析小立碗藓基因组中合成萜类物质的基因,比较小立碗藓与酵母和拟南芥合成萜类物质基因的氨基酸序列同源性同时利用UPLC-QTOF分析小立碗藓中物质组成,来分析小立碗藓基因组中萜类物质合成的基因及小立碗藓中存在的萜类物质。与酵母相比,小立碗藓两条萜类次生代谢途径完整,途径中的基因及氨基酸丰富性更高,提示可以合成更丰富的前体物质如FPP,GPP等;小立碗藓与拟南芥的序列相似性较高,萜类背景简单。UPLC-QTOF分析检测到小立碗藓中次生代谢物质主要是芳香族化合物及各类生物碱,一种萜类物质ent-16beta-Methoxy-19-kauranoic acid。小立碗藓中本身具有合成萜类前体物质和二萜的基因,检测到少量萜类物质,适合作为萜类活性物质异源合成的底盘细胞。

According to the genomic information and KEGG database,we analyzed terpenoids synthetase genes in the genome of the moss of Physcomitrella patens,compared protein sequence homology of terpenoid synthetase in the moss with that of Arabidopsis thaliana and Saccharomyces cerevisiae,analyzed chemical composition of Physcomitrella patens by UPLC-QTOF to explore the gene of terpenoid synthetase and present terpenoid.Compared with Saccharomyces cerevisiae,Physcomitrella patens had two complete terpenoid secondary metabolic pathways.The genes and amino acids in the pathway are more abundant,suggesting that Physcomitrella patens could synthesize more abundant precursors,such as FPP and GPP.The sequence similarity of Physcomitrella patens and Arabidopsis thaliana is higher,and the background of terpenoids in Physcomitrella patens is more simple.UPLC-QTOF analysis showed that the secondary metabolites of Physcomitrella patens are mainly aromatic compounds and alkaloids,and obtained a terpenoid ent-16 beta-Methoxy-19-kauranoic acid.Physcomitrella patens has its own genes for the synthetase of terpene precursors and diterpenoid,and has a small amount of terpenoids,which might be suitable for the heterogenous synthesis of the terpene active substances.