miR-184通过抑制EPB41L5调控大鼠肾癌细胞增殖及迁移

miR-184 might Regulate Proliferation and Migration of Rat Kidney Cancer Cells by Inhibiting EPB41L5

为了探讨miR-184对肾癌细胞的影响及机制,本研究选取肾癌细胞株786-0细胞,随机分为对照组、空白转染组和miR-184转染组,其中miR-184转染组转染miR-184 mimic,空白转染组转染空白mimic,采用CCK-8细胞增殖实验检测各组细胞增殖,流式细胞仪检测各组细胞凋亡,划痕实验检测各组细胞迁移,Western blotting检测各组EPB41L5蛋白表达。研究结果表明miR-184转染组培养48 h和培养72 h时OD值分别为(0.964±0.103)和(1.011±0.121),明显低于对照组和空白转染组(p<0.05);miR-184转染组培养72 h后细胞凋亡率为(18.22±2.26)%,明显高于对照组和空白转染组(p<0.05);miR-184转染组培养24 h后细胞迁移数为(17.21±3.06)个,明显低于对照组和空白转染组(p<0.05);miR-184转染组细胞EPB41L5蛋白相对表达量为(0.241±0.061),明显低于对照组和空白转染组(p<0.05)。本研究初步表明:miR-184可抑制肾癌786-0细胞增殖和迁移,促进细胞凋亡,其可能与其抑制EPB41L5蛋白表达有关。

To investigate of the effect of miR-184 on renal cell carcinoma cells and its mechanism,786-0 cells of renal cell carcinoma cell line were selected and randomly divided into control group,blank transfection group and miR-184 transfection group,respectively.Mi R-184 transfection group was transfected by miR-184 mimic and blank mimic was transfected in blank transfection group.Cell proliferation was detected by CCK-8 cell proliferation assay,apoptosis in each group was detected by flow cytometry,cell migration in each group was detected by scratch test,and EPB41 L5 protein expression in each group was detected by Western blotting.The results showed that miR-184 transfection group cultured 48 h and 72 h OD value respectively were(0.964±0.103)and(1.011±0.121),significantly lower than that of the control group and the blank transfection group(p<0.05);The apoptosis rate of miR-184 transfection group was(18.22±2.26)%after 72 h culture,which was significantly higher than that in the control group and the blank transfection group(p<0.05);The cell migration number of miR-184 transfected group was(17.21±3.06)after 24 h culture,which was significantly lower than that of the control group and the blank transfection group(p<0.05);The relative expression of EPB41 L5 protein in the miR-184 transfected group was(0.241±0.061),which was significantly lower than that in the control group and the blank transfection group(p<0.05).The preliminary results of this study showed that miR-184 could inhibit the proliferation and migration of kidney cancer cells 786-0 and promote apoptosis,which might be related to the inhibition of EPB41 L5 protein expression.