TRPM4在牛心脏快传导系统中的表达

Expression of TRPM4 in cardiac rapide conduction system of bovine

目的检测瞬时受体电位M4型通道(TRPM4)在牛心脏传导系统中的表达状况,为进一步理解TRPM4的病理生理机理提供帮助。方法从牛心脏室间隔肌部起始处中上部连续做多个1cm×1cm切块,分离His束和左右束支,与心尖处及其附近分离浦肯野纤维;使用TRPM4兔抗人抗体和desmin鼠抗人抗体或NAV1.5鼠抗人抗体标记心脏传导系统,马松三色标记传导系统细胞周围的结缔组织。结果TRPM4大量表达于心脏传导系统中的右束支和浦肯野纤维;在共聚焦显微镜下观察到TRPM4不仅表达于牛心脏传导细胞的细胞膜上,而且大量表达于细胞质中。结论TRPM4在心脏传导系统中的大量表达有可能是其引起心脏传导系统疾病的病理生理基础。

Objective To detect the expression of transient receptor potential cation channel subfamily M member 4(TRPM4) in bovine cardiac conduction system, in order to better understand the pathophysiological mechanism of TRPM4. Methods Several 1 cm×1 cm blocks were made from the upper and middle part of bovine ventricular septal muscle to separate His bundle and left and right bundle branches, and Purkinje fibers were separated from the apex and its vicinity. Cardiac conduction system was labeled with TRPM4 rabbit anti-human antibody and mouse anti-human desmin antibody or mouse anti-human NAV1.5 antibody. Peripheral connective tissue was labeled by Marsson tricolor.Results Abundant expression of TRPM4 was detected in the right bundle and Purkinje fibers of cardiac conduction system by immunohistochemistry. Under confocal microscope, TRPM4 was not only expressed on cell membrane of bovine cardiac conduction cells, but also in the cytoplasm. Conclusion The high expression of TRPM4 in the cardiac conduction system may be the pathophysiological basis of cardiac conduction disease.