甜瓜霜霉病苗期抗病性鉴定方法建立及种质资源评价

Establishment of Identification Methods and Germplasm Resource Evaluation of Cucumis melo ssp.melo Downy Mildew at Seedling Stage

【目的】研究甜瓜霜霉病苗期人工接种鉴定的最佳方法,并对甜瓜资源进行苗期接种鉴定,为抗病育种提供抗源。【方法】采用不同接种方法,不同接种苗龄,不同接种浓度三因素三水平正交试验设计,建立甜瓜霜霉病苗期抗性鉴定方法,并对208份新疆哈密瓜资源和国内引进甜瓜资源进行霜霉病抗病性评价。【结果】甜瓜苗期接种霜霉病最好的方法是喷雾法,接种苗龄为2叶期,接种的最适浓度为5×10^3孢子/mL;筛选出免疫甜瓜霜霉病资源5份,高抗资源7份,中抗资源6份。【结论】甜瓜苗期接种霜霉病方法的确立以及抗霜霉病资源筛选为抗病育种奠定了基础。

【Objective】To study the best method of artificial inoculation identification of melon downy mildew at seedling stage,and carry out inoculation identification of melon resources so as to provide resistance source for disease resistance breeding.【Method】Three factors and three levels of orthogonal test design were used in different inoculation methods,different seedling ages,and different inoculation concentrations to establish a method for identifying the seedling resistance of melon downy mildew,and evaluate the disease resistance of 208 Xinjiang Hami melon resources and the introduced melon resources in China.【Result】The best method for melon seedling inoculation with downy mildew was spray method,the seedling age was 2 leaves,and the optimal concentration of inoculation was 5×10^3 spores/mL.Five resources,seven resources with high resistance and six resources with medium resistance were selected.【Conclusion】The establishment of the method of inoculation against downy mildew at seedling stage and the selection of resources against downy mildew laid a foundation for disease resistance breeding.