双参通脉颗粒对大鼠心肌缺血NF-κBp65蛋白表达的影响

Influences of Shuangshen Tongmai Granules on the Expression of NF-κBp65 Protein in Rats with Myocardial Ischemia

目的研究双参通脉颗粒(GSG)对大鼠心肌缺血的干预作用,并探讨其相关机制。方法40只SD大鼠随机分为假手术组(Sham组)、模型组(MIRI组)、合心爽组(DH组)、中药组(GSG组),每组10只。结扎左冠状动脉前降支造模,成功后Sham组、MIRI组应用生理盐水灌胃,DH组、GSG组药物灌胃,4周后,测定各组心功能、病理改变、血清白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α),Western检测NF-κBp65蛋白表达。结果DH组、GSG组与MIRI组比较,心功能明显改善(P<0.05);DH组、GSG组血清IL-1β、IL-6、TNF-α水平明显低于MIRI组(P<0.05),且GSG组低于DH组(P<0.05);DH组、GSG组心肌NF-κBp65蛋白表达明显低于MIRI组(P<0.05),且GSG组低于DH组(P<0.05)。结论GSG可抑制大鼠心肌缺血氧化损伤,其机制可能与IL-1β、IL-6、TNF-α炎症因子释放减少,NF-κBp65蛋白表达抑制有关。

Objective To explore the protective effects of Shuangshen Tongmai Granules(GSG)on myocardial ischemia in rats and its mechanism.Methods Forty SD rats were randomly divided into Sham operation group(Sham),model group(MIRI),diltiazem hydrochloride group(DH),and traditional Chinese medicine group(GSG).The left anterior descending coronary artery were ligatured to established myocardial infarction(AMI)model in rats.The Sham group and MIRI group were administered normal saline via gastric gavage,while DH group and GSG group were administered drugs via gastric gavage.Four weeks later,functional and pathologic changes in the heart and the contents of IL-1β,IL-6,TNF-αin plasma were measured.NF-κBp65 protein levels were measured by western blot analysis.Results Compared with MIRI group,the cardiac diastolic function was significantly improved in GSG and DH treatment group(P<0.05),the plasma levels of IL-1β,IL-6,and TNF-αwere lower(P<0.05),and that in GSG group was lower than that in the DH group(P<0.05).The results of Western blot showed that the protein expressions of NF-κBp65 in GSG group were lower than those in MIRI group(P<0.05),and the GSG group was lower than that in the DH group(P<0.05).Conclusion GSG alleviates myocardial ischemia;injury by reducing oxidative stress.The mechanism may be related to decrease release of IL-1β,IL-6,TNF-αinflammatory cytokines,and inhibition of NF-κBp65 protein expression.