PD-L1和HIF-1α在人脑胶质瘤中的表达及相关性分析

The Expression and Correlation of Programmed Death-Ligand 1 and Hypoxia-Inducible Factor-1α in Human Glioma

目的研究缺氧诱导因子-1α(HIF-1α)和程序性死亡因子配体1(PD-L1)在人脑胶质瘤中的表达及其相关性。方法收集2012年6月—2017年6月于山西省人民医院神经外科行手术治疗后,病理结果证实为胶质瘤病人的石蜡包埋标本43份。采用免疫组织化学法检测脑胶质瘤组织中PD-L1及HIF-1α的表达水平,分析PD-L1与HIF-1α表达水平之间的相关性以及在不同病理级别中的表达情况。结果43例脑胶质瘤组织中PD-L1、HIF-1α在低级别胶质瘤病人中阳性表达低于高级别胶质瘤病人。脑胶质瘤中HIF-1α表达和PD-L1表达呈正相关(r=0.468,P<0.01)。结论PD-L1与HIF-1α在脑胶质瘤组织中明显共表达,表达程度与胶质瘤的恶性程度相关。

Objective To investigate the expression and correlation between the hypoxia-inducible factor-1α(HIF-1α)and programmed death-ligand 1(PD-L1)in glioma.Methods Forty-three paraffin-embedded specimens of glioma patients confirmed by pathological results were collected after surgical treatment.Immunohistochemistry was used to detect the expression levels of PD-L1 and HIF-1αin glioma tissues.The correlation between the expression levels of PD-L1 and HIF-1αproteins and their expression in different pathological grades were analyzed.Results The positive expression of PD-L1 and HIF-1αin 43 glioma tissues was lower in low-grade glioma patients than those in high-grade glioma patients.Spearman correlation analysis showed that there was a positive correlation between HIF-1αexpression and PD-L1 expression in glioma(r=0.468,P<0.01).Conclusion The HIF-1αand PD-L1 proteins are highly co-expressed,and the expression level was correlated with the malignant degree of glioma.