三种何首乌单体成分对大鼠肝损伤作用的研究

Effects of Three Polygonum multiflorum Monomers on Liver Injury in Rats

目的:探究三种何首乌单体对SD大鼠潜在肝脏毒性。方法:雄性SD大鼠随机分为对照组(0.5%羧甲基纤维素钠)及大黄素-8-O-β-D-葡萄糖苷28、280、1120mg·kg^-1剂量组,单蒽酮6.5、65、650mg·kg^-1剂量组,大黄素甲醚6.5、65、650mg·kg^-1剂量组,连续14d经口灌胃给药,观察动物临床症状,分析体质量、肝脏质量、血清生化指标及病理学指标变化,并检测IL-6、IL-10、TNF-α和IFN-γ的表达水平。结果:连续14d给予SD大鼠3种何首乌单体对动物无明显整体毒性和肝脏毒性作用,但与对照组相比,三种单体给药组IL-10水平均降低(P<0.05),大黄素甲醚给药组IL-6和TNF-α水平升高(P<0.05),单蒽酮给药组IFN-γ水平降低(P<0.05)。结论:本研究可见大黄素甲醚存在潜在肝损伤作用。

Objective:To investigate the potential hepatotoxicity effects of three Polygonum multiflorum monomers in SD rats. Methods:Male SD rats were randomly divided into control (0.5% sodium carboxymethyl cellulose ),28,280,1120 mg · kg^-1 of emodin-8- O-β-D -glucoside groups,6.5,65,650 mg · kg ^-1 of anthrone groups and 6.5,65,650 mg · kg^-1 of physcion groups,and administrated through oral gavage for consecutive 14 days.Clinical symptoms,body weight,liver weight,serum biochemical examinations data and histopathological examination results were analyzed,and the levels of IL-6,IL-10,TNF-α and IFN-γ were detected. Results:No definite overall toxicity associated with three monomers was observed in SD rats repeated dosed for consecutively 14 days.However,compared with the control group,the levels of IL-10 were elevated in three medicated groups ( P <0.05 ),the levels of IL-6 and IFN-γ were elevated in physcion groups ( P <0.05 )and the levels of IFN-γ were decreased in anthrone groups ( P <0.05 ). Conclusion:This study demonstrated the potential liver injury caused by physcion.