三明野生黄精不定芽增殖和生根体系研究

Study on Adventitious Bud Proliferation and Inducing Roots System of Sanming Wild Polygonatum sibiricum

[目的]建立三明野生黄精不定芽增殖和生根体系。[方法]以三明野生黄精块茎为材料,研究不同植物生长调节剂对外植体芽增殖、生根的影响。[结果]最佳增殖培养基:MS+1.0mg/L6-BA+1.5mg/LTDZ+0.5mg/L2,4-D+0.5mg/LIAA,增殖系数是3.02;NAA比IBA更有利于诱导三明黄精生根。最佳生根培养基为1/2MS+1.0mg/LNAA+1.0mg/LIBA,生根率为65.0%。[结论]为三明野生黄精工厂化育苗和规模化栽培提供可行的关键技术。

[Objective]To develop the adventitious bud proliferation and inducing roots system for Sanming wild Polygonatum sibiricum.[Method]The rhizomes of Sanming wild P.sibiricum were used to study the influence of different hormones on propagation and rooting.[Result]The best culture medium for proliferation is MS+1.0 mg/L 6-BA+1.5 mg/L TDZ+0.5 mg/L 2,4-D +0.5 mg/L IAA.The proliferation coefficient was 3.02.NAA is more conducive than IBA to induce roots.The optimal rooting medium is 1/2MS+1.0 mg/L NAA+1.0 mg/L IBA,rooting rate was 65.0%.[Conclusion]It provided some feasible techniques for industrialized breeding and scale cultivation of P.sibiricum.