GNM C7-8实时荧光PCR系统对掺假肉制品中多种动物源性成分的同时检测

Simultaneous Detection of Multiple Animal-derived Ingredient in Adulterated Meat Products by GNM C7-8 Real-time Fluorescence PCR System

应用GNMC7-8实时荧光PCR系统,同时快速检测掺假肉制品中多种动物源性成分。将牛肉粉、驴肉粉、鸵鸟肉粉和羊肉粉4组不同肉粉中分别混入猪肉粉、鸭肉粉、马肉粉、鸡肉粉和狐狸肉粉中的2~3种肉粉,制备人工模拟掺假肉制品。通过GNMC7-8实时荧光PCR和ABI7500荧光PCR方法,对上述四种掺假肉制品进行多种动物源性成分检测和比较。结果表明,GNMC7-8实时荧光PCR方法与ABI7500荧光PCR方法从掺假肉制品中检测猪、鸭、马、鸡和狐狸5种成分的循环数一致,分别是31、31、29、32和25,但基于八模块设计的GNMC7-8实时荧光PCR方法能同时检测四种掺假肉制品中不同动物源性成分,检测时间更短。因此,GNMC7-8实时荧光PCR系统的八个模块能够独立运行,互不干扰,无交叉污染,能够实现对掺假肉制品中多种动物源性成分的同时快速检测,为肉制品掺假检测提供强有力的方法支持。

The GNM C7-8 real-time fluorescent PCR system was used to simultaneously and rapidly detect various animalderived ingredients in adulterated meat products.Bovine meal powder,donkey meal powder,ostrich meal powder and ovine meal powder were respectively mixed with two or three kinds of other animal meal powders of the porcine,duck,horse and chicken. Four groups of artificial adulterated meat products were prepared.They were detected and compared with various animal - derived ingredients by GNM C7 - 8 real- time fluorescent PCR and ABI 7500 fluorescent PCR.The results showed that the number of cycles detected by GNM C7 -8 real- time fluorescence PCR for five kinds of ingredients of pigs,ducks,horses, chickens and foxes from the adulterated meat products was the same as that detected by ABI7500 fluorescent PCR method and was 31,31,29,32 and 25 respectively.However,GNM C7 - 8 real - time fluorescent PCR based on eight modules could simultaneously detect different animal - derived components in four adulterated meat products,and the detection time was shorter.Therefore,the eight modules of GNM C7 - 8 real - time fluorescent PCR system could run independently without interference and cross-contamination and realize simultaneously and rapidly detection of various animal-derived ingredients in adulterated meat products,which would provide powerful method support for the detection of adulterated meat products.