基于拉曼光谱快速检测山西老陈醋醋泥中总黄酮方法的建立

Establishment of Rapid Determination of Total Flavonoids in Shanxi Aged Vinegar by Raman Spectroscopy

建立山西老陈醋醋泥中总黄酮拉曼光谱快速检测方法。经乙醇-超声浸提法得到的山西老陈醋粗提液,纯化,采用便携式拉曼仪对总黄酮含量检测,对积分时间、激光功率、积分次数进行优化。结果表明:总黄酮拉曼特征峰在731cm^-1处。最佳检测条件为积分时间:25s;激光功率:350mW;积分次数:5次。其精密度、重现性、稳定性的相对标准偏差值分别为7.82%,4.33%,4.11%,平均加标回收率为98.75%。新建立的山西老陈醋醋泥中总黄酮拉曼光谱快速检测方法达到了一线执法人员检测要求,可以实现现场快速检测。

To establish a Raman rapid detection method for total flavonoids in Shanxi aged vinegar.Crude extract of total flavonoids from Shanxi aged vinegar was obtained by ethanol-ultrasonic extraction,then the crude extract of total flavonoids was further purified.The total flavonoids was detected by a portable Raman instrument.Then the detection conditions were optimized,including integration time,laser power and number of integrations.The results indicated that the Raman characteristic peak of total flavonoids was at 731 cm- 1.The best detection condition were integration time 25 s;laser power 300 mW;the number of integration 5 times.The relative standard deviation ( RSD) values of precision,reproducibility and stability were 7.82%,4.33%,4.11%,and the average spike recovery was 98.75%.The newly established method for rapid detection of total flavonoids in Shanxi aged vinegar meets the requirements of on-site inspection.