基于化合物与蛋白互作分析根皮苷的降糖机制

Hypoglycemic Mechanism of Phloridzin Based on Chemical-Protein Interaction Network

目的:以根皮苷为对象,分析根皮苷在分子层面的降糖机制。方法:通过Stitch和ChEMBL网络数据库检索获得根皮苷的作用靶点及蛋白互作信息,利用分子复合物检测算法(MCODE)对网络进行模块分析及功能注释。结果:从数据库中筛选出21个靶点,构建的蛋白互作网络中有170个节点和545条边,通过聚类分析得到10个功能模板,模块分析表明,根皮苷主要参与组蛋白乙酰化、血糖稳态、乙酰胆碱分解、戊糖降解、嘌呤核苷酸阴离子转运、钙离子稳态等生物过程,发挥降糖作用。结论:本研究从分子网络水平分析了根皮苷的降糖机制,为糖尿病的治疗提供新途径。

Objective:Phloridzin was employed as the research object,and the hypoglycemic effect on molecular level was analyzed.Methods:Phloridzin was chosen in this study to obtain the targets of the components and protein-protein information though Stitch and ChEMBL databases retrieval.Module analysis and function annotation of network were analyzed by a graph theoretic clustering algorithm molecular complex detection( MCODE).Results:21 targets were screened from database.There were 170 nodes and 545 deges in the protein interaction network.10 function modules were obtained from cluster analysis. Hypoglycemic effect of phloridzin was mainly associated with histone acetylation,glucose homeostasis,acetylcholine catabolic process,pentose catabolic process,purine nucleoside transport,anion transport,calcium ion homeostasis.Conclusion:In this study,the anti-diabetes mechanism of phloridzin was elucidated systematically from molecular network level,which provided new approaches for the treatment of diabetes.