燕麦β-葡聚糖含量不同测定方法的适用性和相关性比较

Applicability and Correlation among Different Methods for Content Determination of Oats β-glucan

测定β-葡聚糖含量的方法较多,为探究不同方法的适用性,本文选用3个有代表性的方法,即酶法(国际标准)、凝胶色谱法(不仅可以定量还可以测定分子量)和刚果红法(高通量定量),测定不同来源样品其燕麦β-葡聚糖含量,以比较凝胶色谱法、刚果红法与国际标准酶法的线性相关性、一致性及适用性。结果表明:对于高纯度燕麦β-葡聚糖样品,三种方法测定结果一致性为102%~108%,一致性很好,三种方法均可采用;对于低纯度燕麦β-葡聚糖,凝胶色谱法与酶法的一致性为96%~111%,测定结果存在强线性相关性(r=0.967,p=0.000);刚果红法比酶法测定结果偏高,一致性为143%~228%,测定结果存在线性相关性(r=0.723,p=0.012)。最后比较了三种方法的优势和局限性,各个方法具有无法替代的优势,实际测定中可以从对结果的准确性与实际经济性的比较中选择合适的方法。

Different analysis methods for β-glucan content were reported in literature.Three main methods,i.e.,enzyme method ( an international standard),gel chromatography( both quantitative and molecular weight measuring),and Congo red method( a high throughput way) were compared in different samples to investigate the linear correlation,consistency and applicability of gel chromatography and Congo red as compared with enzyme method.It was concluded that:For high-purity oats β-glucan,any of the three methods could be chosen with good consistency at the level between 102% and 108%.While for low- purity sample,the consistency for gel chromatography compared with enzyme method( an international standard) was 96% to 111%, and significant linear correlation( r = 0.967,p = 0.000) for the results between the two methods was observed.The Congo red method might lead to higher content value with consistency from 143% to 228%,and linear correlation( r = 0.723,p = 0.012) as compared with enzyme method.At last,the merits and drawbacks were compared among three methods,each of which was of irreplaceable advantage.It was suggested that the method which was taken in actural measurement could be considered by comparison of the accuracy and economy.