不同剂量当归口服对硫化钠致小鼠斑秃模型的影响

Effect of Different Doses of Angelica Sinensis on the Alopecia Areata Induced by Sodium Sulfide in Mice

目的:探讨当归口服对硫化钠致小鼠斑秃模型的影响及其可能机制。方法:将50只雄性KM小鼠随机均分为空白对照组、模型组和当归高、中、低剂量组,每组10只。模型组和当归高、中、低剂量组均以8%硫化钠酒精溶液局部外涂建立斑秃模型,当归高、中、低剂量组予以不同剂量当归煎剂灌胃,空白对照组和模型组予以等体积生理盐水灌胃,每天1次,连续7 d。采用酶联免疫吸附(ELISA)和苏木-伊红素(HE)染色法分别检测小鼠血清白细胞介素-10(IL-10)水平和单位视野毛囊数,同时对小鼠背部脱毛区毛发生长情况进行评分。结果:模型组毛发生长评分、单位视野毛囊数及血清IL-10水平均显著低于空白对照组(P<0.05)。与模型组比较,高、中、低剂量组毛发生长评分、单位视野毛囊数及血清IL-10水平均显著高于模型组(P<0.05)。中剂量组组毛发生长评分明显高于高、低剂量组(P<0.05),低剂量组评分与高剂量组毛发生长评分、单位视野毛囊数及血清IL-10水平比较无明显差异(P>0.05)。结论:口服当归对硫化钠致斑秃小鼠模型有生发作用,且以中剂量效果最佳,其生发作用可能与提高血清IL-10水平有关。

Objective: To investigate the effect of Angelica Sinensis on the model of alopecia areata induced by sodium sulfide and its possible mechanism. Methods: Fifty male KM mice were randomly divided into control group, model group and Angelica Sinensis high, medium and low dose groups, with 10 rats in each group. The model group and the high, medium and low doses of Angelica sinensis were locally coated with 8% sodium sulfide alcohol solution to establish alopecia areata model. The high, medium and low doses of Angelica sinensis were given different doses of Angelica sinensis. The blank control group and the model group were given. The same volume of normal saline was administered once a day for 7 days. The levels of serum interleukin-10(IL-10) and the number of follicles per unit of field were measured by enzyme-linked immunosorbent assay(ELISA) and hematoxylin-eusin(HE) staining, respectively. The growth was scored. Results: The hair growth score, the number of hair follicles per unit field and the serum IL-10 level in the model group were significantly lower than those in the blank control group(P<0.05). Compared with the model group, the hair growth score, the number of percutaneous hair follicles and the serum IL-10 level in the high, middle and low dose groups were significantly higher than those in the model group(P<0.05). The hair growth score of the medium dose group was significantly higher than that of the high and low dose groups(P<0.05). There was no significant difference between the low dose group and the high dose group, the hair growth score, the number of percutaneous hair follicles and the serum IL-10 level(P>0.05). Conclusion: Oral Angelica has a germinating effect on sodium sulfide-induced alopecia areal mouse model, and the best effect is moderate dose. The growth of hairy root may be related to the increase of serum IL-10 level.