非小细胞肺癌患者血清miR-146a、miR-141的表达及临床意义

Expression and Clinical Significance of Serum miR-146a and miR-141 in Patients with Non-small Cell Lung Cancer

目的:研究非小细胞肺癌(NSCLC)患者血清微小RNA-146a(mi R-146a)、mi R-141的表达及临床意义。方法:选取2015年1月至2018年1月我院收治的106例NSCLC患者记为NSCLC组,另选取同期于我院进行体检的40例健康体检者记为健康对照组。采用荧光实时定量PCR(qRT-PCR)检测两组血清mi R-146a、mi R-141的表达水平,分析NSCLC患者血清mi R-146a、mi R-141表达水平与临床病理参数的关系,比较血清mi R-146a、mi R-141单一诊断及联合诊断NSCLC的曲线下面积(AUC)、灵敏度、特异度。结果:NSCLC组患者的血清mi R-146a、mi R-141表达水平高于健康对照组(P<0.05)。NSCLC患者血清mi R-146a表达水平与病理分期有关(P<0.05),与年龄、性别、吸烟史、病理类型、病理分级、肿瘤直径及是否伴有淋巴结转移无关(P>0.05)。NSCLC患者血清mi R-141表达水平与病理分期、病理分级及是否伴有淋巴结转移有关(P<0.05),与年龄、性别、吸烟史、病理类型、肿瘤直径无关(P>0.05)。血清mi R-146a、mi R-141单一诊断及两者联合诊断NSCLC的AUC分别为0.841、0.772、0.921,敏感度分别为85.1%、80.5%、93.5%,特异度分别为84.2%、75.3%、89.8%。结论:NSCLC患者血清mi R-146a、mi R-141表达水平升高,且与部分临床病理参数有关,两者联合检测对NSCLC诊断具有较高的诊断价值。

Objective: To study the expression and clinical significance of serum microRNA-146 a(mi R-146 a) and mi R-141 in patients with non-small cell lung cancer(NSCLC). Methods: 106 cases of NSCLC who were treated in our hospital from January 2015 to January 2018 were recorded as NSCLC group, another 40 healthy persons who were examined in our hospital during the same period were recorded as healthy control group. Fluorescence real-time quantitative PCR(q RT-PCR) was used to detect the expression levels of mi R-146 a and mi R-141 in two groups. The relationship between serum mi R-146 a and mi R-141 expression and clinicopathological parameters in NSCLC patients was analyzed. Comparison of the area under curve(AUC), sensitivity and specificity of serum mi R-146 a and mi R-141 for single and combined diagnosis of NSCLC. Results: The serum levels of mi R-146 a and mi R-141 in the NSCLC group were significantly higher than those in the healthy control group(P<0.05). The level of serum mi R-146 a in patients with NSCLC was related to pathological stage(P<0.05). It was not related to age, sex, smoking history, pathological type, pathological grade, tumor diameter and lymph node metastasis(P>0.05). The expression level of serum mi R-141 in NSCLC patients was related to pathological stage, pathological grade and lymph node metastasis(P<0.05). It was not related to age, sex, smoking history, pathological type and tumor diameter(P>0.05).The AUC of serum mi R-146 a and mi R-141 and the combined diagnosis of NSCLC were 0.841, 0.772 and 0.921 respectively. The sensitivity were 85.1%, 80.5% and 93.5% respectively. The specificity were 84.2%, 75.3% and 89.8% respectively. Conclusion: The expression level of mi R-146 a and mi R-141 increased in NSCLC patients, it is related to some clinicopathological parameters. The combined detection of these two methods has high diagnostic value for NSCLC diagnosis.