TGR5和CDX2在胃黏膜肠化生及胃癌中的表达及意义

Expression and Significance of TGR5 and CDX2 in Gastric Mucosal Intestinal Metaplasia and Gastric Carcinoma

目的:探讨G蛋白偶联受体(G protein-coupled receptor,TGR5)和尾型同源盒2(Caudal type homeobox 2,CDX2)在胃黏膜肠化生(Intestinal Metaplasia,IM)及胃癌中的表达和意义。方法:采用免疫组织化学染色法检测TGR5和CDX2在57例慢性胃炎、85例IM、98例胃癌组织中的表达。比较各组间TGR5和CDX2的表达差异,并分析其与胃癌临床病理参数的关系及与胃癌患者预后的关系。利用Spearman秩相关检验分析TGR5和CDX2表达的相关性。结果:IM和胃癌组织中TGR5的高表达率分别为54.1%和58.2%,二者比较无显著差异(P>0.05),但均显著高于慢性胃炎组织(P<0.01)。TGR5高表达与胃癌患者TNM分期(III+IV期)(P=0.004)、淋巴结转移(P=0.046)及预后较差(P=0.006)相关。胃癌组织中CDX2的高表达率(30.6%)较IM组织(48.2%)显著降低(P<0.05),但其均显著高于慢性胃炎组织(P<0.01)。CDX2高表达与胃癌患者TNM分期(I+II期)(P=0.008)、无淋巴结转移(P=0.014)、预后较好(P=0.023)相关。TGR5和CDX2表达在慢性胃炎和IM中无相关性(P>0.05),在胃癌中呈显著正相关(P=0.003)。结论:TGR5和CDX2在IM和胃癌组织中均显著上调,可能参与了IM和胃癌的发生发展。

Objective: To investigate the expression and significance of G protein-coupled receptor(TGR5)and Caudal type homeobox 2(CDX2) in the gastric mucosal intestinal metaplasia(IM) and gastric carcinoma. Methods: Immunohistochemical staining was used to detect the expression of TGR5 and CDX2 in 57 cases of chronic gastritis, 85 cases of IM and 98 cases of gastric cancer. The differences in expression of TGR5 and CDX2 between the groups were compared, and the relationship between the expression of TGR5 and CDX2 and the clinicpatholo-gical parameters and prognosis of gastric cancer patients were analyzed. Spearman rank correlation test was used to analyze the correlation between the TGR5 and CDX2 expression. Results: The high expression rates of TGR5 in the IM and gastric cancer tissue were 54.1% and 58.2%, respectively(P>0.05), but they were significantly higher than chronic gastritis tissue(P<0.01).High expression of TGR5 was associated with TNM stage(III+IV stage)(P=0.004), lymph node metastasis(P=0.046), and worse prognosis(P=0.006) of patients with gastric cancer. The high expression rate of CDX2 in the gastric cancer tissue(30.6%) was significantly lower than that in IM tissue(48.2%)(P<0.05), but both were significantly higher than those in the chronic gastritis tissue(P<0.01). High expression of CDX2 was associated with the TNM stage(I+II stage)(P=0.008), no lymph node metastasis(P=0.014), and better prognosis(P=0.023) in patients with gastric cancer. There was no correlation between TGR5 and CDX2 expression in the chronic gastritis and IM(P>0.05), but they were positively correlated in the gastric cancer(P=0.003). Conclusions: TGR5 and CDX2 are significantly up-regulated in both IM and gastric cancer tissues, and may be involved in the occurrence and development of IM and gastric cancer.