宫颈高级别病变和宫颈癌患者HPV感染状况及基因型分布

Infection status and genotype distribution of human papillomavirus in patients with high-grade cervical lesions and cervical cancer

目的分析宫颈高级别病变和宫颈癌患者HPV感染状况及基因型分布特点。方法采用PCR-反向点杂交法对200例宫颈高级别病变和宫颈癌患者进行HPV基因分型检测。结果检出HPV亚型共计20种,包括16种高危型HPV和4种低危型HPV。其中,HPV 16的感染最常见(55.50%),其次为HPV 52(17.00%)、HPV 58(17.00%)、HPV 33(10.50%)、HPV 18(8.00%)、HPV 31(4.50%)。HPV单一感染140例;其中,HPV 16为80例(57.14%),HPV 58为18例(12.86%)。宫颈上皮内瘤变(CIN)2、CIN3及宫颈癌患者的HPV单一感染率和多重感染率比较差异均无统计学意义(P>0.05)。结论宫颈高级别病变及宫颈癌患者HPV感染的主要亚型依次为HPV 16、HPV 52、HPV 58、HPV 33、HPV 18和HPV 31;九价疫苗对宫颈癌防控意义更大,但仍需扩大样本量进一步调查研究。

Objective To analyze the infection status and genotype distribution of human papillomavirus(HPV) in the patients with high-grade cervical lesions and cervical cancer.MethodsThe genotype of HPV in 200 patients with high-grade cervical lesions and cervical cancer was analyzed by PCR-reverse dot hybridization.Results A total of 20 HPV subtypes was detected,including 16 high-risk HPVs and 4 low-risk HPVs.Statistical analysis showed that HPV 16 was the main infection(55.50%),which was followed by HPV 52(17.00%),HPV 58(17.00%),HPV 33(10.50%),HPV 18(8.00%) and HPV 31(4.50%).Of 140 cases with single HPV infection,HPV 16 occurred in 80 cases(57.14%) and HPV 58 occurred in 18 cases(12.86%).There was no significant difference in the single and multiple infection among the patients with CIN2,CIN3 and cervical cancer(P>0.05).Conclusion The main subtypes of HPV infection in the patients with high-grade cervical lesions and cervical cancer were in an order of HPV 16>HPV 52>HPV 58>HPV 33>HPV 18>HPV 31.The 9-valent HPV vaccine may be important for the prevention and control of cervical cancer,but which needs extensive samples for further investigation.