丁香油对丙酸睾丸酮诱导大鼠前列腺增生的抑制作用

Inhibition of clove oil on benign prostatic hyperplasia induced by testosterone propionate

目的研究丁香油对丙酸睾丸酮诱导大鼠前列腺增生的抑制作用,并探讨其作用机制。方法将60只SD大鼠随机分成对照组、模型组、非那雄胺1mg/kg组和丁香油1、2、4mg/kg组,每组各10只。非那雄胺组和丁香油组大鼠每日ig给药1次,连续给药30d。对照组和模型组ig给予同等体积蒸馏水,检测大鼠前列腺腺体的湿质量、前列腺指数和对大鼠前列腺细胞NADPH氧化酶的影响。并观察非那雄胺10μmol/L和丁香油10、20μmol/L对5α-还原酶的抑制作用。结果与模型组比较,丁香油1、2、4 mg/kg组大鼠前列腺湿质量和前列腺指数均明显降低(P<0.01)。与模型组比较,丁香油1、2、4 mg/kg组的荧光显著降低(P<0.01),说明丁香油对前列腺细胞NADPH氧化酶的活性有明显抑制作用。与非那雄胺组比较,丁香油10、20μmol/L组对5α-还原酶活性的平均Ⅰ均显著升高(P<0.01),说明丁香油对5α-还原酶的活性有明显抑制作用。结论丁香油具有明显抑制前列腺增生的作用,其机制主要是通过抑制5α-还原酶和抑制NADPH氧化酶的作用。

Objective To study the inhibiting effects of clove oil on benign prostatic hyperplasia induced by testosterone propionate and its mechanism. Methods SD rats(60) were randomly divided into control group, model group, finasteride 1 mg/kg group, and clove oil 1, 2, and 4 mg/kg group, and each group had 10 rats. Rats in finasteride group and clove oil group were ig administered with drugs for 30 d, once daily. The control group and model group were ig administered with the same volume of distilled water. Prostate wet mass and index of rat and effects of clove oil on NADPH oxidase in prostate cells of rat were detected. The inhibiting effects of finasteride 10 μmol/L and clove oil 10 and 20 μmol/L on 5α-reductase were observed. Results Compared with the model group, the wet mass of the prostate and the prostate index in clove oil 1, 2, and 4 mg/kg group were significantly reduced(P < 0.01). Compared with the model group, fluorescence in clove oil 2 and 4 mg/kg group were significantly reduced(P < 0.01), indiicating that clove oil had the inhibiting effects on NADPH oxidase in prostate cells. Compared with the finasteride group, mean Ⅰ of clove oil 10 and 20μmol/L on 5α-reductase were significantly increased(P < 0.01), indiicating that clove oil had the inhibiting effects on 5α-reductase in prostate cells. Conclusion Clove oil has significant effect on inhibiting benign prostatic hyperplasia, and its mechanism of action is mainly through inhibition of 5α-reductase and inhibition of NADPH oxidase.