亚麻木酚素对AAPH诱导的红细胞和肝组织氧化应激的保护作用

Protection effect of flax lignan on AAPH-induced oxidative stress in erythrocytes and liver tissue

探讨了亚麻木酚素的抗氧化活性及机制,测定了亚麻木酚素对DPPH自由基的清除能力,建立AAPH氧化损伤大鼠红细胞和肝组织模型,研究了亚麻木酚素对氧化损伤红细胞溶血率和血红蛋白氧化率的影响,以及其对红细胞和肝组织氧化损伤模型丙二醛(MDA)和抗氧化酶的影响。结果显示:亚麻木酚素对DPPH自由基具有清除作用;亚麻木酚素对红细胞溶血和血红蛋白氧化的保护作用呈现浓度依赖性,与AAPH作用4 h的模型组相比,50μmol/L和100μmol/L亚麻木酚素的保护作用显著提高;在AAPH诱导的红细胞氧化应激模型中,100μmol/L和150μmol/L亚麻木酚素显著降低了AAPH损伤4 h时MDA含量,并显著提高了GSH-Px的酶活,150μmol/L亚麻木酚素显著增加了AAPH损伤2 h和4 h时的SOD和CAT的酶活;在AAPH诱导的肝组织模型中,150μmol/L亚麻木酚素能有效降低不同AAPH损伤时间(1~4 h)所增加的MDA含量,增加AAPH损伤4 h时降低的SOD和CAT酶活。说明亚麻木酚素具有抗氧化活性,能够抑制脂质过氧化,保护红细胞和肝组织内抗氧化酶系的活性。

In order to study the antioxidant activity and mechanism of flax lignan,its scavenging ability on DPPH free radical was determined,and the erythrocytes and liver tissue models of AAPH-damaged rats were established to research the effects of flax lignan on hemolysis rate,hemoglobin oxidation rate,malondialdehyde (MDA) and antioxidant enzymes. The results showed that flax lignan had a scavenging effect on DPPH free radical. The protective effect of flax lignan on erythrocyte hemolysis and hemoglobin oxidation was concentration-dependent,and compared with the model group treated with AAPH for 4 h,the protective effects of 50 μmol/L and 100 μmol/L flax lignan were significantly improved. In the AAPH-induced erythrocytes oxidative stress model,100 μmol/L and 150 μmol/L flax lignan remarkably reduced the MDA content in model group treated with AAPH for 4 h, and significantly increased the activity of GSH-Px. 150 μmol/L flax lignan significantly increased the activities of SOD and CAT in the group damaged with AAPH for 2 h and 4 h. In liver tissue damaged by AAPH,flax lignan (150 μmol/L) reduced the MDA content with different damageable time (1-4 h),and increased SOD and CAT enzymes activities when AAPH damaged for 4 h. It indicated that flax lignan had antioxidative effect,and it could inhibit peroxidation of lipids,and protect antioxidative enzymes activities in erythrocytes and liver tissue.