Triple-TOF-MS/MS法检测植物油中甘油三酯

Determination of triglyceride in vegetable oil by Triple-TOF-MS/MS

建立了一种高分辨质谱(Triple-TOF-MS/MS)测定芝麻油、葵花籽油、大豆油、菜籽油、花生油和玉米油等常见食用植物油中甘油三酯的方法,分析并确定不同食用植物油中每种碳原子当量(ECN)下的甘油三酯组成与含量。结果表明:采用Triple-TOF-MS/MS法可以根据一级质谱母离子(加氢母离子、加钠离子和加氨母离子)相对分子质量以及二级质谱的碎片离子([DAG]^+)对相同ECN的甘油三酯进行定性分析。采用该方法对芝麻油、葵花籽油、大豆油、菜籽油、花生油和玉米油进行甘油三酯检测,结果共获得35种甘油三酯,均含有LnLL、LLL、LLO、PLL、LOO、PLO和OOO 7种甘油三酯。

A method for determining triglyceride in common edible vegetable oils such as sesame oil,sunflower seed oil,soybean oil,rapeseed oil,peanut oil and corn oil by Triple-TOF-MS/MS was established. And the compositions and contents of triglycerides of different ECNs in different vegetable oils were analyzed and determined. The results showed that Triple-TOF-MS/MS method could be used to qualitatively analyze triglycerides of the same ECN based on the relative molecular weight of the primary mass spectrometer precursor (hydrogenation precursor ion,sodium addition ion and ammonia precursor ion) and the fragment ion ([DAG]^+) pair of the secondary mass spectrometer. A total of 35 triglycerides were detected in sesame oil,sunflower seed oil,soybean oil,rapeseed oil,peanut oil and corn oil by the method. All the six oils contained seven triglycerides LnLL,LLL,LLO,PLL,LOO,PLO and OOO.