淡水中叶绿素a的实时检测设计

Design of Real-time Detection of Chlorophyll-a in Freshwater

本文研制了一种基于水中叶绿素a在激光照射下可以发出荧光的特性来测定水中叶绿素a含量的方法,采用脉冲式高亮发光二极管作为光源,发出的光经过470nm的滤光片照射到被测水体,水体中的叶绿素a受到激发后,发出比激发光波长更长的荧光,荧光强度与叶绿素的浓度在一定范围内存在线性关系,发出的荧光被光电池接收并产生电信号,根据电信号的强弱来计算被测水体的叶绿素a含量变化。该方法具有免试剂、可移动、实时检测的特点,非常适用于我国大面积长距离水源地水质的安全预警监测和应急监测。

In this paper, a method based on the fluorescing characteristics of chlorophyll a in water to determine the chlorophyll a content in water is developed. The pulsed high-brightness lightemitting diode is used as the light source, and the emitted light is irradiated to the measured by a 470nm filte匚 In the water body, the chlorophyll a in the water body is excited, and emits fluorescence longer than the wavelength of the excitation light. The fluorescence intensity and the concentration of chlorophyll have a linear relationship within a certain range, and the emitted fluorescence is received by the photo cell and generates an electric signal according to the electric signal. Strength and weakness to calculate the change of chlorophyll a content in the measured water The method has the characteristics of reagent-free, movable and real-time detection, and is very suitable for safety early warning monitoring and emergency monitoring of large-area long-distance water source water quality in China.