不同钾水平对人参中核苷类化合物含量的影响

Effects of Different Potassium Levels on Nucleosides and Bases in Panax ginseng

应用建立的超高效液相色谱法(UPLC)同时测定人参中13种核苷类化合物含量的方法,研究不同施钾水平条件(1,3,6,9,12 mmol/L)下人参中核苷类化合物含量的差异。采用超声法提取,UPLC测定聚炔类化合物的含量,色谱条件为Waters HSS T3(100 mm×2. 1 mm,1. 8μm)色谱柱,以乙腈(B)-0. 01%甲酸水为流动相进行梯度洗脱(1~8 min,0~8%B; 8~9 min,8%~15%B; 9~10. 5 min,15%B; 10. 5~11 min,15%~0%B; 11~15 min,0%B),流速为0. 3 m L/min,检测波长为260 nm,在此条件下13种组分达到基线分离,且线性范围内均具有良好的线性关系。试验结果表明:不同钾水平条件下人参中13种核苷类化合物的含量差异较大,核苷类化合物总含量为1 908. 74~2 527. 89μg/g,随着钾水平的提高,核苷类化合物总含量呈现出先降低后升高的趋势;黄嘌呤、尿苷、鸟苷、2’-脱氧鸟苷4种核苷类最为丰富,其中黄嘌呤含量随着钾浓度的升高呈现逐渐升高的趋势,最高含量为324. 55μg/g;尿苷和鸟苷的含量则呈现先降低后逐渐升高的趋势,钾浓度为6 mmol/L时二者含量最低,分别为309. 97μg/g和156. 00μg/g,钾浓度为12 mmol/L时尿苷最高含量为741. 71μg/g,不施钾的对照组鸟苷的最高含量为637. 27μg/g; 2’-脱氧鸟苷的含量随着钾水平的提高呈现降低的趋势,含量为529. 53~227. 60μg/g。UPLC方法操作简便、准确可靠、重复性高,可用于人参中核苷类化合物含量的测定。不同施钾水平条件下人参中核苷类化合物含量的差异较大,可为人参生产中钾肥的使用提供依据。

The study was designed to establish the method of simultaneous determination of the content of 13 nucleosides compounds in Panax ginseng by ultra-high performance liquid chromatography(UPLC),and to compare the differences of nucleosides in ginseng under different potassium levels(1,3,6,9,12 mmol/L),which will provide basis for ginseng production. The nucleosides and bases in ginseng were extracted through water with ultrasonic assisted and determined by UPLC,and the chromatographic column was Waters HSS T3(100 mm × 2. 1 mm,1. 8 μm). The mobile phase was acetonitrile(B) and 0. 01% acid water using a gradient program(1-8 min,0-8% B; 8-9 min,8%-15% B; 9-10. 5 min,15% B; 10. 5-11 min,15%-0% B,11-15 min and 0% B) at the flow rate of 0. 3 m L/min. Chromatograms were recorded at 260 nm. There was a greater difference between the content of nucleosides and bases in ginseng under different potassium level conditions. The total nucleosides and bases content were at the range of 1 908. 74-2 527. 89 μg/g. With the increase of potassium level,the trend of nucleosides and bases was decreased and then increased. Four nucleosides and bases, including xanthine, uridine, guanosine and 2’-deoxyguanosine,are the most abundant in the ginseng. The content of adenine was increased with the increase of potassium level,and the highest content was 324. 55 μg/g; the trend of uridine and guanosine was decreased and then increased,and the lowest content was 309. 97 and 156. 00 μg/g,respectively,at K level of 6 mmol/L. The highest content of uridine(12 mmol/L K) and guanosine(ck,0 mmol/L) was 741. 71 μg/g and 637. 27 μg/g,respectively. The trend of the content of 2’-deoxyguanosine was decreased with the increased K level. The established UPLC method is simple,accurate and reliable,and it could be used for the content determination of thirteen nucleosides in ginseng. The content of nucleosides is obviously different in ginseng under different potassium levels,and it could provide the basis for the reasonable potassium application of ginseng.