NK、Th17和Treg细胞水平对结直肠癌患者术后复发预测价值的分析

Analysis on Predictive Value of NK, Th17 and Treg Cells on Recurrence of Colorectal Carcinoma after Laparoscopic Resection

目的:探讨NK、Th17和Treg细胞水平对经腹腔镜根治术后的结直肠癌患者临床结局的影响,并分析其预测结直肠癌患者的复发的价值。方法:纳入2016年1月—2017年1月行腹腔镜根治术治疗的原发结直肠癌患者120例进行随访。采集术后第一天外周血检测NK、Th17和Treg细胞数量,采用两独立样本t检验分析其术后复发和未复发患者间差异;利用logistic回归模型判断各指标与复发的关联;最后采用ROC曲线分析其在预测结直肠癌术后复发的价值。结果:术后复发组NK和Th17细胞水平显著低于未复发组(P<0.05),但Treg细胞水平复发组要高于未复发组(P<0.05)。经Logistic回归分析发现,NK细胞和Th17细胞是复发的保护性因素,而Treg细胞是独立的危险因素。ROC曲线分析表明,NK、Th17和Treg细胞水平对预测术后复发具有一定的预测价值。结论:结直肠癌患者其复发与NK、Th17和Treg细胞水平有关,是结直肠癌患者术后预后的独立影响因素,其对结直肠癌患者预后具有一定的影响,预测患者术后复发具有一定的价值。

Objective To investigate the influences of the levels of NK cells, Th17 cells and Treg cells on clinical outcomes of patients with colorectal carcinoma after laparoscopic resection, and to analyze their predictive value for postoperative recurrence. Methods The clinical data were collected from 120 patients with colorectal carcinoma who underwent laparoscopic resection bfrom January, 2016 to January, 2017. The levels of NK cells, Th17 cells and Treg cells in peripheral blood were obtained and detected on day 1 postoperatively. Two independent t-test samples were used to analyze the difference between patients with recurrence and those without recurrence. Logistic regression model was used to determine the correlation between the indicators and recurrence. Finally, ROC curve was used to analyze its value in predicting recurrence of colorectal cancer after operation. Results We found that levels of NK cells and Th17 cells in recrudesce patients were lower than those in non-recrudesce patients (P <0.05), but the level of Treg cells in recrudesce patients were higher than that in non-recrudesce patients (P <0.05). Logistic regression analysis found that NK cells and Th17 cells were protective factors for recurrence, while Treg cells were independent risk factors. ROC curve analysis showed that the levels of NK cells, Th17 cells and Treg cells had certain predictive value for predicting postoperative recurrence. Conclusion Levels of NK cells, Th17 cells and Treg cells are associated with recurrence and independent prognostic influencing factors for postoperative patients with colorectal carcinoma, and its has a certain value in predicting postoperative recurrence of patients.