MTHFR C677T位点，ABCG2 G34A位点基因型与Ⅳ期结肠腺癌无进展生存期的关系

Association of single nucleotide polymorphisms of MTHFR C677T and ABCG2 G34A with progression-free survival of stage Ⅳ colon adenocarcinoma

目的 分析亚甲基四氢叶酸还原酶(MTHFR)、三磷酸腺苷结合转运蛋白G超家族第2成员(ABCG2)基因单核苷酸多态性(SNP)与Ⅳ期结肠腺癌预后的关系。方法对2011年1月至2015年1月收治的137例Ⅳ期结肠腺癌患者进行回顾性分析。检测患者外周血MTHFR C677T位点,ABCG2 G34A位点的SNP,随访统计患者无进展生存期(PFS)。分析MTHFR、ABCG2的SNP与患者预后的关系。结果 MTHFR C677T位点野生型42例,突变型95例;ABCG2 G34A位点野生型63例,突变型74例。单独分析显示上述位点突变与患者PFS无明显关系;综合分析中,以MTHFR C677T位点野生型和ABCG2 G34A突变型为优势基因型,具备2个优势基因型的患者中位PFS明显长于具备0~1个优势型患者,差异有统计学意义( P <0.05)。Cox回归分析显示优势基因型个数不是患者PFS的独立影响因素( P >0.05)。结论 MTHFR、ABCG2的SNP与Ⅳ期结肠腺癌预后有一定关系,但具备优势基因型并不是患者预后的独立影响因素。

Objective To analyze the relationship between single nucleotide polymorphism (SNP) of methylenetetrahydrofolate reductase (MTHFR),ATP-binding cassette sub-family G member 2 (ABCG2) gene and prognosis of stage IV colon adenocarcinoma.Methods From January 2011 to January 2015,137 patients with stage IV colon adenocarcinoma were analyzed retrospectively. The SNPs of MTHFR C677 T and ABCG2 G34A in peripheral blood were detected,and PFS was followed up,the relationship between the SNPs of MTHFR and ABCG2 and the prognosis of patients were analyzed. Results accounted for 42 cases,with mutant type accounted for 95 cases. Patients with wild type of ABCG2 G34A accounted for 63 cases,with mutant type accounted for 74 cases. Individual analysis showed that there was no significant relationship between the above mutations and PFS. In comprehensive analysis,the dominant genotypes were MTHFR C677 T wild type and ABCG2 G34A mutations. The median PFS of patients with 2 dominant genotypes was significantly longer than that of patients with 0-1 dominant genotype (P < 0. 05). Cox regression analysis showed that the number of dominant genotypes was not an independent factor of PFS (P > 0. 05). Conclusion SNPs of MTHFR and ABCG2 is related to the prognosis of stage IV colon adenocarcinoma,but the dominant genotype is not an independent factor affecting the prognosis of patients.