摘要
To identify the pathogen that causes scuticociliatosis in farmed-raised turbot Scophthalmus maximus , we isolated a ciliate from the brain tissue of an infected turbot and identifi ed it as Uronema marinum based on morphological and molecular evidence. We then infected the turbots in artifi cial laboratory settings with pure cultured U . marinum . The infected turbots showed syndromes similar to those observed in naturally infected ones. Furthermore, microscopic examination and PCR detection confi rmed the presence of the ciliate in the tissues of those laboratory-infected turbots. To our best knowledge, this is the fi rst report of scuticociliatosis caused by U . marinum in farm-raised turbot S . maximus in China.
To identify the pathogen that causes scuticociliatosis in farmed-raised turbot Scophthalmus maximus,we isolated a ciliate from the brain tissue of an infected turbot and identified it as Uronema marinum based on morphological and molecular evidence.We then infected the turbots in artificial laboratory settings with pure cultured U.marinum.The infected turbots showed syndromes similar to those observed in naturally infected ones.Furthermore,microscopic examination and PCR detection confirmed the presence of the ciliate in the tissues of those laboratory-infected turbots.To our best knowledge,this is the first report of scuticociliatosis caused by U.marinum in farm-raised turbot S.maximus in China.
基金
Supported by the National Key R&D Program of China(No.2017YFC1404504)
the National Basic Research Program of China(973 Program)(No.2015CB755904)
the Scientific and Technological Innovation Project financially supported by Qingdao National Laboratory for Marine Science and Technology(Nos.2015ASKJ02,2016ASKJ14)
the Laboratory for Marine Fisheries Science and Food Production Processes,Qingdao National Laboratory for Marine Science and Technology(No.2016LMFS-B08)
