N-N二甲基甲酰胺/纳米银修饰电极的制备与应用研究

Preparation and Application Study of N-N Dimethylformamide/Nano-silver Modified Electrode

用N-N二甲基甲酰胺和纳米银对导电玻璃(ITO)进行修饰得到了N-N二甲基甲酰胺/纳米银修饰电极。研究了电极的电化学行为,在pH=6.64的PBS缓冲溶液中,盐酸二甲双胍在0.075~0.2mg/mL浓度范围内与氧化峰电流呈现很好的线性关系,线性方程为y=317.19x+30.076,最低检出限为0.000 7mg/mL,线性相关线性系数R2为0.959 5。平行测量5组数据,其RSD值为0.75%,在盐酸二甲双胍缓释片溶液中5次加标回收率平均值为101%。精密度和准确度的分析结果表明方法可应用于药物中盐酸二甲双胍含量的测定。

N-N dimethylamide/nano-silver modified electrode was used to modify conductive glass(ITO)with N-N dimethylformamide and nano-silver.The electrochemical behavior of the electrode was studied.In PBS with pH 6.64 as buffer solution,metformin hydrochloride showed a good linear relationship with oxidation peak current within the concentration range of 0.075-0.2 mg/mL while linear equations was y=317.19 x+30.076,and the minimum detection limit was 0.000 7 mg/mL.The correlation linear coefficient r squared is 0.959 5.Parallel measurement of 5 sets of data with an RSD value of 0.75%.In metformin hydrochloride sustained-release tablet solution,the average recovery rate was 101%after 5 times of standard addition.The results of precision and accuracy analysis showed that this method can be applied to the determination of metformin hydrochloride in medicine.