p21 codon31基因多态性与宫颈癌的关系

Relationship between p21 codon31 gene polymorphism and cervical cancer

目的探讨p21codon31基因单核苷酸多态性与中国汉族人群宫颈癌统计学、发病风险及临床病理学参数的关系。方法选取2014年1月—2016年6月陕西西安及湖南地区汉族宫颈癌患者178例作为研究组,并选取相同时间和地点的237例健康群众作为对照组。采用限制性片段长度多态性分析(PCRRFLP)对p21codon31基因多态性基因分型。对两组基因型和临床病理学参数进行分析。结果对照组中p21codon31基因Ser等位基因频率高于研究组(P<0.05),研究组中p21codon31基因Ser/Ser基因型频率高于对照组(P<0.05),p21codon31基因Ser等位基因携带者患宫颈癌风险是Arg等位基因携带者的1.345倍(P<0.05),p21codon31基因Ser/Ser基因型携带者患宫颈癌风险是Arg/Arg基因型携带者的2.01倍(P<0.05)。结论p21codon31基因多态性与中国汉族人群宫颈癌的发病风险有关系,其中携带p21codon31基因Ser/Ser基因型人群有更高的宫颈癌的患病风险。

Objective To investigate the association between the p21 codon31 polymorphism and statistics, cervical cancer risk and clinicopathological characteristics in Chinese Han population. Methods Totally 178 cases of cervical cancer from January 2014 to June 2016 in Xi’an and Hunan provinces of Shaanxi Province were selected as the study group,and 237 healthy people at the same time and place were selected as the control group. The polymorphism of p21 codon31 gene was typed by restriction fragment length polymorphism(PCR-RFLP). Genotypes and clinicopathological parameters of the two groups were analyzed. Results Ser allele frequency of p21 codon31 gene in control group was higher than that in research group(P < 0.05). Ser/Ser genotype frequency of p21 codon31 gene in research group was higher than that in control group(P < 0.05). Ser allele carriers of p21 codon31 gene had 1.345 times higher risk of cervical cancer than those of Arg allele carriers(P < 0.05). Ser/Se of p21 codon31 gene was 1.345 times higher than that of Arg allele carriers(P < 0.05).The risk of cervical cancer in carriers of R genotype was 2.01 times higher than that in carriers of Arg/Arg genotype(P < 0.05). Conclusions The polymorphism of p21 codon31 gene is associated with the risk of cervical cancer in Chinese Han population. Ser/Ser genotype carriers of p21 codon31 gene have higher risk of cervical cancer.