金属蛋白酶对大菱鲆血清抗氧化酶活性及丙二醛含量的影响

Effects of metalloprotease on antioxidant system enzyme activities and malondialdehyde content in the serum of Scophthalmus maximus

为了研究金属蛋白酶对大菱鲆(Scophthalmus maximus)抗氧化系统的影响,作者分别对大菱鲆肌肉注射不同浓度的金属蛋白酶,并于3、6、12、24、48、72h测定血清中谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)、总超氧化物歧化酶(T-SOD)、总抗氧化活力(T-AOC)以及丙二醛(MDA)含量变化。结果显示,低质量浓度组(0.037mg/mL)金属蛋白酶处理后,CAT和T-SOD活力除3h显著高于对照组外(P<0.05),其余时间点均与对照组无显著差异(P>0.05),而低浓度组GSH-Px和T-AOC在整个试验过程中均与对照组无显著性差异(P>0.05)。中质量浓度组(0.073mg/mL)金属蛋白酶胁迫后血清中CAT、GSH-Px、T-SOD和T-AOC在3h^72h均呈现波浪形的变化趋势。高质量浓度组(0.147mg/mL)金属蛋白酶对上述4种抗氧化酶产生不同程度的抑制,且在6h^72h均显著低于对照组(P<0.05)。金属蛋白酶低质量浓度和中质量浓度组MDA含量呈现较为平稳的变化趋势,且与对照组无显著性差异(P>0.05),而高质量浓度组MDA含量在3h^72h均显著高于对照组(P<0.05)。

A metalloprotease was purified from the extracellular products (ECP) of Vibrio anguillarum. It was lethal to S. maximus when injected intramuscularly. However, there is little information about the effects of metalloprotease on the antioxidant system and lipid peroxidation in S. maximus. In this study, we measured catalase (CAT), glutathione peroxidase (GSH-Px), total antioxidation activity (T-AOC), total superoxide dismutase (T-SOD) activity, and malondialdehyde (MDA) content in the serum of S. maximus exposed to 0.037 mg/mL, 0.073 mg/mL, or 0.147 mg/mL metalloprotease, respectively, at 3, 6, 12, 24, 48, and 72 hours. The results showed that compared with the control group, the activities of CAT and T-SOD were not significantly different at any of the tested time points, except for 3 h (P > 0.05). The variation in GSH-Px and T-AOC was not obvious in the serum of the low-concentration group. The changes in CAT, GSH-Px, T-SOD, and T-AOC in the serum of the medium-concentration group after metalloproteinase stress undulated between 3 h and 72 h. Metalloproteinase in the high-concentration group was inhibited by the above four antioxidant enzymes and was significantly lower than in the control group from 6 h to 72 h. The MDA content of the low- and medium-concentration groups was not significantly different between 3 h and 72 h (P > 0.05), while the MDA content of the high-concentration group was significantly higher than that of the control group from 3 h to 72 h (P < 0.05).