一株新城疫病毒F基因的克隆及其遗传进化分析

Cloning and genetic evolution analysis of one strain of newcastle disease virus

从新乡市某养鸡场的疑似新城疫病鸡群中分离到1株新城疫病毒,并对该病毒分离株进行F基因的扩增、序列测定和遗传进化分析。结果表明,该新城疫病毒分离株的F基因全长为1662bp,F蛋白裂解位点区的氨基酸序列为112R-R-Q-K-R-F117,具备强毒株的特征;同源性分析表明,该新城疫病毒分离株与国内外流行的基因Ⅶ型强毒株之间的同源性较高,为87.5%~97.7%,其中与国内流行的基因Ⅶd型的参考毒株同源性最高,为95.4%~97.7%,而与现用疫苗株LaSota、Clone-30、B1、Mukteswar等同源性较低,84.2%~86.6%;系统进化树分析表明,该新城疫病毒分离株与基因Ⅶd型参考毒株FJ882014、FJ608335和GU227738处在同一个进化分支上,亲缘关系最近。

A Newcastle disease virus(NDV) was isolated from ND suspected chickens of a scale chicken farm in Xinxiang. The F gene of the isolated Newcastle disease virus(NDV) was amplified and analyzed by genetic evolution. The results showed that the whole nucleotied sequence length of F gene was 1 662 bp. The sequence of amino acid of F gene at the cleavage site is 112R-R-Q-K-R-F117, which is consistent with the characteristics of strong NDV strain. Comparison of the NDV with the strong NDV of gene Ⅶ of epidemic strains from different countries, results showed that the nucleotide homology was 87.5%~97.7%, and in which the comparison with the Ⅶd was 95.4%~97.7%. Comparison of the NDV isolated with the current vaccine strains such as La Sota, Clone-30, B1, Mukteswar and so on, the nucleotide homology was 84.2%~86.6%. The phylogenetic tree analysis demonstrated that the NDV isolated and Ⅶd strains of FJ882014,FJ608335 and GU227738 were located in the same cladogram branch.