自然发酵驼乳对乙醇诱导的小鼠急性肝损伤的保护作用

Protective Effects of Naturally Fermented Camel Milk on Acute Alcohol-Induced Liver Injury in Mice

研究自然发酵驼乳对小鼠急性酒精性肝损伤的保护作用。将24只雄性ICR小鼠随机分为正常组、模型组和自然发酵驼乳组。连续灌胃两周后,给小鼠灌胃50%乙醇溶液(7.3 g/kg)建立肝损伤模型。测定各组小鼠体质量、肝脏指数以及血清和肝脏的相关生化指标;将肝脏组织进行HE染色,观察肝组织病理学变化。结果显示:与对照组比较,模型组血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)的活性以及促炎细胞因子的含量显著增加(p<0.05或p<0.01),肝组织病理切片显示出明显的损伤和大量炎性细胞浸润。与模型组相比,自然发酵驼乳组ALT活性显著下降(p<0.05),肿瘤坏死因子-α(TNF-α)及白细胞介素-1β(IL-1β)含量极显著降低(p<0.01)。HE染色的结果显示小鼠肝组织中炎性细胞浸润明显减少,脂肪变性程度减轻。肝脏丙二醛(MDA)含量显著下降(p<0.05),超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性显著升高(p<0.05),肝脏甘油三酯(TG)含量极显著降低(p<0.01)。结果表明,自然发酵驼乳对小鼠急性酒精性肝损伤有较好的保护作用,这可能与降低小鼠体内炎症应答水平和改善肝脏氧化应激程度有关。

To study the protective effects of naturally fermented camel milk on acute alcoholic liver injury in mice. 24 male SPF CD-1(ICR)mice were randomly divided into 3 groups,the normal control group,model control group,and naturally fermented camel milk group. After a two week administration,the acute alcohol liver injury mouse model was set up by intragastric administration of alcohol in the dose of 7.3 g/kg. Body weight,liver index,serum and liver biochemical indicators were measured in each group.The pathologicalchanges of liver were observed by light microscope with HE staining. The result showed that,compared with the control group,activities of serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),and proinflammatory cytokines contents of serum in the model group were significantly increased(p<0.05 or p<0.01). The liver histopathological sections also showed significant injury and extensive inflammatory cell infiltration. Compared with the model group,the ALT activities of the naturally fermented camel milk group decreased significantly(p<0.05),and the contents of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)decreased significantly(p<0.01). The results of HE staining showed that the inflammatory cell infiltration in the liver tissue was significantly reduced,and the degree of steatosis was reduced. The contents of malondialdehyde(MDA)in the liver of naturally fermented camel milk group was significantly decreased(p<0.05),the activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)was significantly increased(p<0.05),and the contents of TG was significantly lower than that of the model group(p<0.01).The results show that naturally fermented camel milk has a good protective effect on acute alcoholic liver injury in mice,which exert its beneficial effects mainly through reducing the contents of inflammatory response in body and improving the oxidative stress in the liver of mice.