表面增强拉曼光谱用于食品检测的研究进展

Research Progress on Application of Surface Enhanced Raman Spectroscopy in Food Detection

表面增强拉曼光谱法(surface enhanced raman spectroscopy,SERS)是一种新型光谱分析技术,其原理是基于被测分子吸附在某些经过特殊处理、具有纳米结构的金属表面具有极强拉曼散射增强效应的分子振动光谱技术,具有操作简便,灵敏度高、原位取样、无损检测等优点,故广泛应用于食品品质安全检测。本文综述了SERS技术在食品中药物残留、防腐剂、色素、甜味剂等方面的研究,SERS技术还可结合化学计量学、量子力学理论、光学等领域知识对某些物质进行定量和定性研究,简要概述了SERS技术在大分子构象方面的研究,以及诸如生物毒素、掺伪掺假等其他方面的研究。SERS信号相对较弱,易受食品基质以及荧光效应的干扰,故SERS技术与其他分离技术的整合,是未来的重点方向之一。SERS技术研究食品加工过程中某些物质的转化产物、代谢物、反应物以及杂质,也将是SERS分析的热点之一,故SERS是食品检测一个极具潜力的工具。

Surface enhanced raman spectroscopy as a spectral analysis technique based on the molecules under test was adsorbed on certain specially treated,nanostructured metal surfaces with strong Raman scattering enhancement to characterize the molecular vibrational spectroscopy technology,had the advantages of simple operation,high sensitivity,in-situ sampling and non-destructive testing,so it was widely used in food quality safety detection.This paper reviews the research progress of SERS technology in the detection of drug residues,preservatives,pigments,sweeteners,and can also be used to combine with other disciplines such as chemometrics,quantum mechanics theory,optics to quantitatively and qualitatively study certain substances.A brief overview of SERS technology in the study of macromolecular conformation,as well as other aspects such as biotoxins,adulteration and adulteration are summarized.The SERS signal is relatively weak and is susceptible to interference from food matrix and fluorescence effects. Therefore,the integration of SERS technology with other separation technologies will be one of the key directions. SERS technology study the conversion products,metabolites,reactants and impurities of certain substances in food processing. It will also be one of the hotspots of SERS analysis,so SERS is a promising tool for food testing.