摘要
目的本文采用高效液相色谱-蒸发光散射法(HPLC-ELSD),分析各种模拟回收油的差异,从而进行回收油的鉴别。方法将常用的大豆油、花生油和动物油脂的样品混合后模拟回收油脂,采用丙酮提取后直接进样分析,色谱柱条件为C18非极性柱,流动相为二氯甲烷-乙腈(体积比30∶70),流速1.0mL/min,采用蒸发光散射检测器检测,保持柱温35℃,使各种甘油三酯分离,对比处理各样品谱图数据。结果混入了动物油脂的植物油与其纯品植物油两者的甘油三酯的组成变化较大。结论通过分析油脂中甘油三酯组成特征获取油脂种属信息,进而能够对疑似从餐厨垃圾中回收提炼的涉假(劣质)植物油进行鉴别。
Objective To identify the recovery oil by analyzing the differences of various simulated recovery oils with high performance liquid chromatography-evaporative light scattering (HPLC-ELSD). Methods The simulated recovery oil, blended with soybean/peanut oil and animal grease, was extracted by acetone. The extract was undergone through HPLC- ELSD analysis under the conditions: C 18 nonpolar column, keeping its temperature of 35 C, being owed with mobile phase of dichloromethane -acetonitrile (30:70 V/V) at running rate of 1.0 mL/min, and evaporative light scattering detector for gauging. The separated triglycerides were compared of their spectrums against the spectral data from various original constituent oil. Results Triglyceride composition varied greatly when pure vegetable oil mixed with animal grease. Conclusions The analysis of triglyceride components can identify the quality-inferior/adulterated vegetable oil suspected to be re ned from recovered food waste.
作者
乔杰
时秋娜
赵鹏程
QIAO Jie;SHI Qiuna;ZHAO Pengcheng(Henan Police College, Zhengzhou 450046, China;China Criminal Investigation Police University, Shenyang 110845, China)
出处
《刑事技术》
2019年第4期317-321,共5页
Forensic Science and Technology
基金
河南警察学院院级课题(No.HNJY-2018-44)
关键词
甘油三酯
回收油
高效液相-蒸发光散射法
triglyceride
recovered oil
high performance liquid phase - evaporative light scattering method
