硬膜外腔持续液体灌注对兔硬膜外腔压力及液体分布的影响

The effects of constant-irrigation in epidural space on the epidural pressure and fluid distribution in rabbits

目的研究兔硬膜外腔液体灌注对硬膜外腔压力、颅内压及对液体在硬膜外腔上升高度的影响。方法选用新西兰兔36只,均在颅顶及下腰部建立颅内压测压通道、硬膜外腔测压通道(L5/6)、硬膜外腔灌注通道(L6/7)。将实验动物随机分为六组,每组6只,利用蠕动泵控制灌注流速。A组(未灌注)、B组(0.1mL/min生理盐水灌注)、C组(0.5mL/min生理盐水灌注)、D组(1.0mL/min生理盐水灌注)、E组(0.5mL/min碘海醇灌注)、F组(1.0mL/min碘海醇灌注)。分别记录未灌注组、各生理盐水灌注组在T0(0min)、T1(2min)、T2(4min)、T3(8min)时间点时硬膜外腔压力及颅内压,并记录两碘海醇灌注组造影剂在灌注容量为R1(1mL)、R2(2mL)、R3(4mL)时的上升节段。结果硬膜外腔压力及颅内压随灌注时间增加而升高,随后到达平台期。随着灌注速度增加,平台期硬膜外压力及颅内压峰值也随之增加,B、C、D组三组间差异有统计学意义(P<0.05)。在相同灌注量下,硬膜外液体上升高度与速度之间无明显相关性(P>0.05)。结论硬膜外压力及颅内压灌注平台期峰值与灌注速度呈正相关性,与灌注时间(量)无明显相关性。灌注液体上升高度与灌注速度无明显相关性,与灌注时间(量)呈正相关性。

objectiveTo observe the changes of pressure and intracranial pressure in epidural cavity of rabbits at different perfusion rates by perfusing the fluid into the epidural cavity. Methods36 New Zealand rabbits were used in this experiment. All the rabbits were established intracranial pressure channels at the top of cranium and lower waist. The experimental animals were randomly divided into six groups with 6 animals in each group. The peristaltic pump was used to control the perfusion flow rate. Group A was without perfusion. Group B was with 0. 1 ml / min normal saline perfusion. Group C was with 0.5ml/min saline infusion. Group D was with 1.0ml/min saline infusion. Group E was with 0.5ml/min iodine infusion. Group F was with 1.0ml/min iodizol infusion group. The epidural pressure and intracranial pressure were recorded at the time of T0(0 min), T1(2 min), T2( 4 min) T3 (8)min) T4 (5 min after perfusion) in each normal saline group. The ascending segments of contrast media in R1(1ml), R2 (2ml), R3(4ml)group were recorded. ResultsThe epidural pressure and intracranial pressure increased with the increase of perfusion time, and then reached the plateau stage. With the increase of perfusion rate, the peak value of epidural pressure and intracranial pressure also increased, and there was statistical difference among the three groups ( P < 0.05). There was no significant correlation between the rising height and velocity of epidural fluid under the same perfusion volume ( P > 0.05). ConclusionThe peak value of epidural pressure and intracranial pressure was positively correlated with perfusion velocity, but had no significant correlation with perfusion time (volume). There was no significant correlation between the rising height of perfusion fluid and the perfusion velocity, but a positive correlation with the perfusion time (volume).