肝癌穿刺组织中抑癌基因Tg737的表达和功能

Study on expression and functions of tumor suppressor gene Tg737 in puncture tissue of hepatocellular carcinoma

目的探讨抑癌基因Tg737在肝癌穿刺组织中的表达状况及功能。方法行肝穿刺获取肝癌组织和邻近正常组织,通过免疫组化和病理切片法,确认组织的性质。对MHCC97-H和SMMC7721细胞株予以培养,将不同浓度的Tg737表达质粒与转染剂配合。按0、1.0、10.0μg/L不同质粒浓度分组,观察Tg737过表达后肝细胞活力状况及VEGF含量。结果较癌旁正常组织,Tg737的蛋白表达量和mRNA表达量在肝癌组织中居更低水平,差异有统计学意义(P<0.05)。转染Tg737表达质粒可使MHCC97-H和SMMC7721细胞株所具有的CCK-8吸光值降低,质量浓度呈越大显示时,吸光值越低;其亦可使MTT吸光值降低,质粒浓度呈越大显示时,吸光值呈越低显示。质量浓度呈越大显示时,VEGFA、VEGFB含量呈越低显示。结论肝癌组织中分布的抑癌基因Tg737表达呈明显减少显示;通过增加Tg737表达,细胞活力降低,VEGF含量、原癌基因表达均显示明显减少。

ObjectiveTo investigate the expression and function of tumor suppressor gene Tg737 in puncture tissues of liver cancer. MethodsLiver puncture was performed on hepatocarcinoma tissues and adjacent normal tissues. As a biopsy specimen, the nature of the tissue was confirmed by immunohistochemistry and pathological sectioning. SMMC7721 and MHCC97-H cell lines were cultured. The Tg737 expression plasmid was transfected with different concentrations. The cell viability and VEGF content after Tg737 overexpression were observed by different plasmid concentrations of 0μg/L, 1.0μg/L and 10.0μg/L. ResultsThe protein expression and mRNA expression of Tg737 in normal tissues adjacent to the cancer were lower in the liver cancer tissues ( P <0.05). Transfection of Tg737 expression plasmid reduced the absorbance of CCK-8 in MHCC97-H and SMMC7721 cell lines. The higher the mass concentration, the lower the absorbance. The transfection of Tg737 expression plasmid enabled MHCC97-H and SMMC7721 cells. The MTT absorbance value of the strain decreased, and the larger the plasmid concentration, the lower the absorbance value. The higher the mass concentration, the lower the VEGFA and VEGFB content. ConclusionFor the clinically treated patients with liver cancer, the expression of the tumor suppressor gene Tg737 distributed in liver cancer tissues was significantly reduced. By increasing the expression of Tg737, the cell viability was decreased, and the expression of VEGF and protooncogene were significantly reduced..