腹主动脉双夹闭法构建大鼠脊髓缺血再灌注损伤的有效性探讨

Study on the validity of abdominal aorta double-clamping method for constructing spinal cord ischemia-reperfusion injury in rats

目的 探讨腹主动脉双夹闭法制作大鼠脊髓缺血再灌注损伤模型的有效性。方法 成年SD大鼠随机分为空白组(Sham组)和缺血再灌注组(SCII组),Sham组大鼠仅开腹不夹闭动脉,SCII组大鼠双夹闭腹主动脉30min。2组大鼠分别在6h、12h、24h、48h进行BBB评分和髓过氧化物酶检测,并在再灌注24h时行HE染色及检测cleaved -caspased -3蛋白水平。结果 与空白组相比,SCII组大鼠BBB评分明显降低,MPO活性及cleaved- caspase- 3蛋白水平明显升高,差异有统计学意义( P <0.05)。HE染色可见SCII组脊髓灰质出现大量的变性神经元,炎性细胞浸润。结论 通过双夹闭腹主动脉可以成功建立大鼠脊髓缺血再灌注损伤模型。

Objective To investigate the effectiveness of abdominal aorta double clamping method in the rat model of spinal cord ischemia reperfusion injury. Methods Adult Sprague Dawley rats were randomly divided into the blank group (Sham group) and the ischemia reperfusion group (SCII group).The Sham group only had open laparotomy without clamping the artery.The SCII group had double clamping of the abdominal aorta for 30min.BBB score and myeloperoxidase were detected at 6h,12h,24h,and 48h,respectively.HE staining and detection of cleaved caspased 3 protein levels were performed at 24h after reperfusion. Results Compared with the blank group,the BBB scores of the SCII group were significantly decreased,and the MPO activity and cleaved caspase 3 protein levels were significantly increased ( P <0.05).HE staining showed that a large number of degenerated neurons and inflammatory cell infiltration appeared in the gray matter of the SCII group. Conclusion The rat spinal cord ischemia reperfusion injury model can be successfully established by double clamping of the abdominal aorta.