精子DNA碎片、弹性硬蛋白酶及精液参数与孕早期流产相关性的前瞻性研究

Prospective Study on the Relationship between Sperm DNA Fragments,Elastoproteinase and Semen Parameters and Early Pregnancy Abortion

目的:探讨孕早期流产是否与精子DNA碎片化指数(DFI)、精浆弹性硬蛋白酶、精子形态、精子动力学参数有关。方法:选取2016年12月-2019年2月于本院生殖中心行人工授精(IUI)或体外受精胚胎移植(IVF-ET)的男性患者120例,根据术后配偶妊娠情况,将其分为IUI分娩组、IUI流产组、IVF分娩组、IVF流产组,各30例。比较各组精子浓度、前向运动精子百分率、精子形态学检查结果、精浆弹性硬蛋白酶、精子DFI。结果:IUI流产组和IUI分娩组精子浓度、前向运动精子百分率、正常形态精子百分率比较,差异均无统计学意义(P>0.05);IVF流产组和IVF分娩组精子浓度、前向运动精子百分率、正常形态精子百分率比较,差异均无统计学意义(P>0.05);IUI流产组、IVF流产组精浆弹性硬蛋白酶及精子DFI均分别高于IUI分娩组、IVF分娩组,差异均有统计学意义(P<0.05)。结论:孕早期流产与精浆弹性硬蛋白酶、精子DFI高有关,与精子浓度、前向运动精子百分率、正常形态精子百分率无关。

Objective:To explore whether early pregnancy abortion is related to sperm DNA fragmentation index(DFI),seminal plasma elastoproteinase,sperm morphology and sperm dynamics parameters.Method:A total of 120 male patients undergoing artificial insemination(IUI)or in vitro fertilization and embryo transfer(IVFET)in the reproductive center of our hospital from December 2016 to February 2019 were selected.According to the pregnancies of spouses after operation,they were divided into IUI delivery group,IUI abortion group,IVF delivery group and IVF abortion group,30 cases in each group.The sperm concentration,forward motility sperm rate,sperm morphology,seminal plasma elastoproteinase and sperm DFI in four groups were compared.Result:There were no significant differences between IUI abortion group and IUI delivery group in sperm concentration,forward motility sperm rate and normal morphology sperm percentage(P>0.05).There were no significant difference between IVF abortion group and IVF delivery group in sperm concentration,forward motility sperm rate and normal morphology sperm percentage(P>0.05).The seminal plasma elastoproteinase and sperm DFI in IUI abortion group and IVF abortion group were higher than those of IUI delivery group and IVF delivery group respectively,the differences were statistically significant(P<0.05).Conclusion:Early pregnancy abortion is associated with high seminal plasma elastoproteinase and sperm DFI,but not with sperm concentration,sperm motility and normal sperm percentage.