通元针法对慢性不可预知温和应激抑郁症模型大鼠行为学的影响

Effect of Tongyuan Acupuncture Method on Behavior of Chronic Unpredictable Moderate Stress Depression Rats

目的:观察通元针法对慢性不可预知温和应激抑郁症模型大鼠行为学的影响,探索针刺干预抑郁症的临床优化方案。方法:SD大鼠60只,按照处理方法不同分成空白对照组10只,造模组50只。空白对照组不予处理,造模组采用慢性不可预知温和应激法进行造模。选造模成功大鼠40只,按照随机数字表法分成模型对照组、帕罗西汀组、常规针刺组、通元针法组,每组10只;模型对照组予生理盐水灌胃,帕罗西汀组每日予帕罗西汀灌胃,常规针刺组电针百会、印堂,通元针法组电针百会、大椎、气海、关元;采用体质量、糖水偏好实验、旷场实验、悬尾实验评价干预治疗效果。结果:与空白对照组比较,造模后各组大鼠体质量均显著减轻、糖水偏好度均显著降低、中央区域停留时间均显著缩短、悬尾不动时间均显著延长(P<0.05)。干预2周后,帕罗西汀组、常规针刺组、通元针法组大鼠各项指标与模型对照组比较,差异均有统计学意义(P<0.05),常规针刺组与通元针法组大鼠的糖水偏好度、中央区域停留时间、悬尾不动时间比较,差异均有统计学意义(P<0.05),帕罗西汀组与通元针法组的大鼠悬尾不动时间比较,差异有统计学意义(P<0.05)。结论:帕罗西汀、常规针刺、通元针法均能有效改善抑郁症模型大鼠的抑郁症状,通元针法对观测指标的改善效果更加明显。

Objective:To observe the effect of tongyuan acupuncture on behavior of chronic unpredictable mild stress depression model rats.Method:60 SD rats were divided into blank control group(n=10)and model group(n=50)according to different treatment methods.The blank control group was not treated,and chronic unpredictable mild stress method was adopted in the model group.40 rats were selected for successful modeling,and were divided into model control group,Paroxetine group,routine acupuncture group and tongyuan acupuncture group according to random number table method,with 10 rats in each group.Normal saline was given to the model control group and Paroxetine was given to the paroxetine group daily,routine acupuncture group electroacupuncture baihui,yintang,tongyuan acupuncture group electroacupuncture baihui,dazhui,qihai,guanyuan.The effects of intervention were evaluated by body mass,sugar water preference test,open-field test and tail suspension test.Result:Compared with the blank control group,the body mass of the rats in each group were significantly reduced after modeling,the preference degree of sugar and water were significantly reduced,the stay time in the central region were significantly shortened,and the tail suspension time were significantly prolonged(P<0.05).After 2 weeks of intervention,all the indicators of the Paroxetine group,the conventional acupuncture group and the tongyuan acupuncture group were compared with the model control group,the differences were statistically significant(P<0.05);comparison of sugar water preference,central region residence time and tail suspension time between the conventional acupuncture group and the tongyuan acupuncture group showed statistically significant differences(P<0.05),comparison of tail suspension time between Paroxetine group and tongyuan injection group showed statistically significant difference(P<0.05).Conclusion:Paroxetine,routine acupuncture and tongyuan injection can effectively improve the depression symptoms of the depression model rats,and tongyuan injection has a more obvious effect on the improvement of the observation indexes.