Hsa-miR-10a-5p靶基因预测及生物信息学分析

Target gene prediction and bioinformatic analysis of has-miR-10a-5p

利用生物信息学对miR-10a-5p的靶基因进行预测及相关分析,为miR-10a-5p靶基因的实验验证及其调控机制提供理论基础。通过 miRBase获取并分析人、大鼠,小鼠等物种的miR-10a-5p的碱基序列特征;使用在线数据库 Targetscan7.1,miRDB,mirDIP和DIANA TOOLS等预测miR-10a-5p的靶基因,并用Venny 2.1绘制韦恩图取交集。用在线工具DAVID对交集靶基因进行GO功能注释和KEGG pathway分析。结果表明 miR-10a-5p成熟序列在各物种间高度保守。获得的79个靶基因在分子功能上主要涉及染色质结合,转录活性激活等,并显著富集于肝脏发育,脂肪组织发育等生物学过程,涉及cAMP信号通路,TNF信号通路及AMPK信号通路。miR-10a-5p通过调控靶基因参与了生命活动和疾病过程中多个方面,尤其生长发育和癌症过程,为进一步研究提供了生物学基础。

To provide theoretical basis for its experimental verification and regulation study, the target genes of miR-10a-5p were predicted and analyzed. The base sequence characteristics of miR-10a-5p of human, rat, mouse, and other animals were obtained and analyzed by miRBase database. The target genes of miR-10a-5p were predicted using the online databases Targetscan7.1, miRDB, mirDIP, and DIANA TOOLS, and the intersection of Wayne maps was drawn using Venny 2.1. The GO function annotation and KEGG pathway analysis were performed on the intersection target genes using the online tool DAVID. The base sequence characteristics of miR-10a-5p of human, rat, mouse, and other species were obtained and analyzed by miRBase. Results show that the mature sequence of miR-10a-5p was highly conservative among different species. There were 79 target genes enriched in molecular function such as chromatin binding and transcription factor activity. These target genes were also involved in the biological development process of liver development and adipose tissue development, and they were mainly located in cellular signaling including cAMP pathway, TNF pathway, and AMPK pathway. In conclusion, the miR-10a-5p is involved in many parts of biological process and pathological process, especially the development process and carcinogenesis, which provides a biological basis for further research.