茶多酚功能化的还原型氧化石墨烯对蛋白质的富集

Adsorption of proteins by tea polyphenols functionalized and reductive graphene oxide

目的研究茶多酚功能化的还原型氧化石墨烯(TPG)对蛋白质的富集,并应用于对唾液中蛋白质的分离富集。方法采用水热法得到了TPG,使用分子荧光来考察TPG对牛血清白蛋白(BSA)及唾液中的蛋白质的富集效果。结果成功制备了TPG,且低浓度(0.10mg·mL^-1)TPG溶液在10min内对模型蛋白BSA的富集效率超过90%,最高吸附容量可达3.874g·g^-1;TPG对实际样本唾液中蛋白质的富集效率高达86%。结论TPG对模型蛋白质及实际样本中的蛋白质都表现出了良好的富集能力,为蛋白质的富集提供了一种高效的新型碳质材料,在蛋白质的研究检测中具有良好的应用潜力。

OBJECTIVE To study the enrichment of proteins from model protein and saliva by tea polyphenols functionalized and reductive graphene oxide (TPG). METHODS TPG was prepared by hydrothermal method and used to enrich the proteins. And the enrichment effect of BSA and proteins in saliva was studied by molecular fluorescence. RESULTS TPG was successfully prepared and showed excellent adsorption ability as high as above 90% within 10 min even at low concentration of TPG (0. 10 mg·mL^-1 ),and showed an efficient enrichment capacity of bovine serum albumin ( BSA) as high as 3. 874 g·^-1. For the proteins in saliva, TPG also showed high adsorption percentage as high as 86%. CONCLUSION TPG has been investigated to be a good material with high enrichment efficiency for BSA and proteins from saliva. TPG is successfully applied in the enrichment of proteins with high efficiency, indicating its good application potential in the protein research.