藏边大黄的质量标准研究

Study on the quality standard of Rheum emodi

目的建立藏边大黄的质量标准。方法采用原植物鉴别、性状鉴别、显微鉴别、理化鉴别四大经典鉴别法鉴别藏边大黄;按2015年版《中国药典》附录中的方法测定藏边大黄的干燥失重、总灰分和浸出物;采用HPLC法测定药材中指标性成分藏黄苷的含量。结果四大鉴别均具显著特征,可作为藏边大黄药材的鉴别方法;并初步拟定了藏边大黄中水分的含量不得过15.0%,总灰分的含量不得过10.0%,浸出物的含量不得少于25.0%,藏黄苷A的含量不得少于3.0%。结论所用方法结果准确,重复性、稳定性均较好,可为藏边大黄药材质量标准的制定提供实验依据。

OBJECTIVE To establish the quality standard of Rheum emodi Wall.. METHODS The origin identification, morphological identification, microscopic identification, physical and chemical identification were carried out. Loss on drying, total ash and extract were determined according to the approaches listed in the Chinese Pharmacopoeia published in 2015. HPLC was employed to determine the content of piceatannol -4'-O-β-D-glucopyranoside in R. emodi. RESULTS The four identification methods all had remarkable characteristics. The limit of moisture and total ash must be less than 15% and 10%, respectively. The extractive and the content of piceatannol-4'-O-β-D-glucopyranoside was more than 25. 0% and 3. 0%, respectively. CONCLUSION The method are accurate, reproducible and stable, which can provide an experimental basis for the quality standards of R. emodi.