一测多评法同时测定梅翁退热颗粒中8种成分

Simultaneous determination of eight constituents in Meiweng Tuire Granules by QAMS

目的建立一测多评法同时测定梅翁退热颗粒(金银花、野菊花、连翘等)中木犀草苷、木犀草素、东北贯众素、连翘酯苷B、连翘酯苷A、连翘苷、牛蒡子苷元、坡模酸的含有量。方法该药物70%甲醇提取液的分析采用Thermo ODS C18色谱柱(250 mm×4.6 mm,5μm);以乙腈-0.5%磷酸为流动相,梯度洗脱;体积流量0.9 mL/min;柱温25℃;检测波长205、275、295、350 nm。以连翘苷为内标,计算其他7种成分相对校正因子,测定其含有量。结果 8种成分在各自范围内线性关系良好(r>0.999 0),平均加样回收率96.98%~100.08%,RSD 0.60%~1.42%。一测多评法所得结果与外标法接近。结论该方法稳定可靠,可用于梅翁退热颗粒的质量控制。

AIM To establish a quantitative analysis of multi-components by single-marker ( QAMS) method for the simultaneous content determination of luteolin-7-glucoside,luteolin,dryocrassine,forsythoside B,forsytho- side A,forsythin,arctigenin and pomolic acid in Meiweng Tuire Granules ( Lonicerae japonicae Flos,Chrysanthemi indici Flos,Forsythiae Fructus,etc.). METHODS The analysis of 70% methanol extract of this drug was per- formed on a 25 ℃ thermostatic Thermo ODS C18 column ( 250 mm×4. 6 mm,5 μm),with the mobile phase com- prising of acetonitrile-0. 5% phosphate acid flowing at 0. 9 mL /min in a gradient elution manner,and the detection wavelengths were set at 205,275,295,350 nm. Forsythin was used as an internal standard to calculate the relative correction factors of the other seven constituents,after which the content determination was made. RESULTS Eight constituents showed good linear relationships within their own ranges ( r>0. 999 0),whose average recoveries were 96. 98%-100. 08% with the RSDs of 0. 60%-1. 42%. The results obtained by QAMS approximated those ob- tained by external standard method. CONCLUSION This stable and reliable method can be used for the quality control of Meiweng Tuire Granules.