梣酮对LPS介导MH7A细胞增殖和迁移的影响

Effects of fraxinellone on LPS-mediated MH7A cell proliferation and migration

目的研究梣酮对LPS介导类风湿关节炎滑膜成纤维细胞系MH7A增殖和迁移的影响。方法 MH7A细胞随机分为对照组、LPS组及梣酮低、中、高剂量组(10、20、50μmol/L),各梣酮组预给药后LPS诱导MH7A细胞24 h。然后,CCK-8法检测细胞活性,试剂盒法检测LDH释放,BrdU掺入法检测细胞增殖,Transwell实验检测细胞侵袭和迁移,Western blot法检测MMP1、MMP9、MMP13、p-JNK、p-STAT3蛋白表达。结果梣酮对MH7A细胞活性、LDH释放无明显影响(P>0.05)。与LPS组比较,梣酮组显著抑制细胞增殖、侵袭和迁移(P<0.05),显著降低MMP1、MMP9、MMP13、p-JNK、p-STAT3蛋白表达(P<0.05),并呈剂量依赖性。结论梣酮可抑制LPS介导MH7A细胞增殖和迁移,可能是通过调控JNK、STAT3信号通路实现的。

AIM To study the effects of fraxinellone on LPS-mediated proliferation and migration of rheumatoid arthritis fibroblast-like synoviocyte line, MH7 A.METHODS MH7 A cells were randomly divided to control group, LPS group and low-dose, medium-dose, high-dose fraxinellone groups(10, 20, 50 μmol/L). All fraxinellone groups pre-treated with various concentrations of fraxinellone were exposed to 24 h LPS induction. Subsequently, cell viability was detected by CCK-8 method, LDH release was detected by kit assay, cell proliferation was detected by BrdU incorporation assay, cell invasion and migration were detected by Transwell assay, MMP1, MMP9, MMP13, p-JNK and p-STAT3 protein expressions were detected by Western blot.RESULTS Fraxinellone demonstrated no obvious effects on MH7 A cell viability and LDH release(P>0.05). Compared with the LPS group, all fraxinellone groups exhibited significantly inhibited cell proliferation, invasion and migration(P<0.05), and markedly decreased MMP1, MMP9, MMP13, p-JNK and p-STAT3 protein expressions(P<0.05) in a dose-dependent manner.CONCLUSION Fraxinellone can inhibit LPS-mediated MH7 A cell proliferation and migration, which may be achieved by regulating JNK and STAT3 signaling pathways.