摘要
免疫荧光技术是标记免疫技术中应用最为广泛的一项技术,具有特异性强、灵敏度高、速度快和便于观察等特点。影响免疫荧光染色质量的因素多种多样,从荧光染料、抗体、样品前处理、染色过程等几个重要的方面入手,就如何提高免疫荧光染色质量,以获得一张高分辨率的图片做了详细的介绍。该研究有利于提高免疫荧光染色质量,为生命科学研究提供了有力帮助。
As one of the most widely used immunolabeling techniques,immunofluorescence has several advantages including high specificity,high sensitivity,rapid detection and clear positioning. However,the quality of immunofluorescent staining could be affected by multiple factors,such as fluorescent dyes,antibodies,sample preparation and staining process.Starting with those vital factors,we introduced how to improve the quality of immunofluorescent staining so as to obtain a high-resolution image in detail.This study improved the quality of immunofluorescent staining and provided powerful help for life science research.
作者
宋瑞龙
周子彦
徐天祺
潘士锋
刘宗平
SONG Rui-long;ZHOU Zi-yan;XU Tian-qi(College of Veterinary Medicine,Yangzhou University/Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou,Jiangsu 225009)
出处
《安徽农业科学》
CAS
2019年第16期124-126,141,共4页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金项目(31502128,31672620,31702204)
江苏省自然科学基金青年基金项目(BK20150447)
江苏高校优势学科建设工程资助项目
关键词
免疫荧光染色技术
抗体
影响因素
Immunofluorescent cell staining technology
Antibody
Influencing factor