黔产青钱柳中总三萜类化合物的含量测定方法优化及其产地加工研究

Optimization of Content Determination of Total Triterpenoids in the Cyclocarya paliurus (Batal.) Iljinskaja of Guizhou and Its Origin Processing Research

[目的]测定黔产青钱柳在不同炮制温度及不同生长年限下总三萜含量的变化,以确定青钱柳产地加工的最佳温度和生长年限对其品质影响的差异。[方法]通过正交试验,样品用30倍量的70%乙醇,超声提取40min,显色后,在546.5nm的波长下测定其吸光度。以齐墩果酸为标准品,测定其总三萜的含量。[结果]齐墩果酸浓度在0.0041~0.0246mg/mL与吸光度呈良好的线性关系(r=0.9997),平均加样回收率为99.55%,RSD为2.24%(n=6),该方法可靠。不同干燥温度和生长年限对黔产青钱柳中总三萜含量的影响具有显著差异,干燥温度在80℃时,总三萜的含量高于其他炮制温度下的,并且高温和低温条件下,青钱柳的色泽加深,影响到成品的外观性状。[结论]青钱柳的最佳干燥温度为80℃,由于不同生长年限中总三萜含量的差异无规律可寻,有待进一步研究确定。

[Objective]The research aimed to determine the change of total triterpenoid content in different processing temperatures and different growth period of Cyclocarya paliurus, and to determine the difference in the optimum temperature and growth period of the origin processing of Cyclocarya paliurus.[Method]By orthogonal experiment,the sample was extracted with 70% ethanol of 30 times,ultrasonic extraction of 40 min, and then the absorbance was determined at the wavelength of 546.5 nm.The oleanolic acid was used as a standard to determine the total triterpenoid content.[Result]The c ontent of the oleanolic acid showed a better linear relationship with the absorbance in the range of 0.004 1- 0.024 6 mg/mL( r =0.999 7).The average recovery was 99.55%,RSD was 2.24%(n =6).The method was reliable.Different drying temperature and growth period of triterpenoid content in Cyclocarya paliurus had significant difference,when the influence of drying temperature was 80 ℃,the content of total triterpenes was higher than other processing temperature,the colour and lustre of Cyclocarya paliurus deepen at high and low temperature conditions,the impact to the finished product appearance.[Conclusion]The best drying temperature of Cyclocarya paliurus was 80 ℃.Because the difference of total triterpenoid content in different growth years is irregular,it needs further research and determination.