摘要
随着高通量测序技术的发展,一种高效而经济的SNP(single nucleotide polymorphism)发掘和基因分型技术GBS(genotyping by sequencing)被开发出来,避免了传统基因型鉴别方法的不足,现已被广泛应用于植物遗传图谱构建、全基因组关联分析、基因多样性研究以及种质鉴定和品种识别等多个领域。从GBS技术的原理、流程、特点以及应用等方面对近年来植物应用GBS技术所进行的研究进展进行综述,并提出了今后GBS技术的研究重点,以期为GBS技术在植物的应用研究提供参考。
With the development of high throughput sequencing technology,GBS ( genotyping by sequencing) technique,an economic technique to discover SNP and genotype myriad of crop germpasms in an effective way,has been developed,which avoids the deficiency of traditional genotypic identification methods.It has been widely used in many fields including construction of genetic map,genome-wide association study,genetic diversity and germplasm dentification and variety identification.In this paper,the research progress of GBS technology in plants was reviewed from the principle,process,characteristics and application.And the research emphasis on GBS technology was putting forward in the future,which was expected to provide reference for the future research of GBS technology on plants.
作者
乔岩
孙佳庆
魏如倩
刘越
QIAO Yan;SUN Jiaqing;WEI Ruqian;LIU Yue(Key Laboratory of Ethnomedicine,Ministry of Education,Minzu University of China,Beijing 100081;College of Life and Enviironmental Sciences,Minzu University of China,Beijing 100081;National Resource Center for Chinese Materia Medica,China Academy of Traditional Chinese Medicine,Beijing 100700,China)
出处
《中国农业科技导报》
CAS
CSCD
北大核心
2019年第8期47-55,共9页
Journal of Agricultural Science and Technology
基金
国家民委中青年英才项目(2016-3-01)
国家自然科学基金项目(81274185,31161140345)
高等学校学科创新引智计划项目(111-08044)
中央民族大学一流大学一流学科建设项目(YLdxxk201819)
中央民族大学创新班项目(SHSY2016120021)资助
教育部新世纪优秀人才支持计划项目(NCET-13-0624)
民族医药教育部重点实验室(中央民族大学)自主课题项目(KLEM-ZZ201807)
国家大学生创新训练计划项目(GCCX2018110022)
关键词
GBS技术
植物
应用领域
研究进展
GBS technology
plant
application field
research progress