基于尿pH研究麻黄对高尿酸血症模型大鼠血尿酸的影响

Effect of Ephedra on Serum Uric Acid in Hyperuricemia Rats Based on Uric pH

目的研究麻黄改善高尿酸血症(HUA)模型大鼠血尿酸(SUA)代谢作用与尿液pH的关系。方法 30只雄性SD大鼠采用随机数字表分为正常组、模型组、别嘌醇组、麻黄组及黄柏组,每组6只。采用灌胃次黄嘌呤联合腹腔注射氧嗪酸钾方法制备大鼠HUA模型,造模成功后麻黄组以麻黄水煎液20mL·kg^-1·d^-1灌胃、黄柏组以黄柏水煎液20mL·kg^-1·d^-1灌胃、别嘌醇组以混悬液20mL·kg^-1·d^-1灌胃,正常组和模型组予等体积蒸馏水灌胃,定时定量给药7天后处死大鼠取材,测定大鼠肝脏指数(LI)、肾脏指数(KI)、SUA、血肌酐(SCr)、24h尿量和尿pH。结果与正常组比较:模型组和别嘌醇组大鼠KI较大[(1.10±0.07)%,(1.07±0.04)%比(0.90±0.05)%,P<0.05],麻黄组和黄柏组大鼠KI显著增加[(1.28±0.17)%,(1.18±0.14)%比(0.90±0.05)%,P<0.01];模型组大鼠血SUA水平明显升高[(77.96±19.40)比(40.66±16.77),P<0.01],别嘌醇组、麻黄组和黄柏组大鼠血SUA无差异[(46.89±9.90)mmol/L、(42.05±8.23)mmol/L、(51.13±13.86)mmol/L比(40.66±16.77)mmol/L,P>0.05];模型组大鼠尿量明显增加[(29.50±5.25)mL比(7.00±2.30)mL,P<0.01],别嘌醇组和黄柏组尿pH增加[(7.55±0.83),(7.63±0.73)比(6.52±0.92),P<0.05],麻黄组大鼠尿pH明显升高[(7.67±0.58)比(6.52±0.92),P<0.01]。与模型组比较:麻黄组大鼠KI较高[(1.28±0.17)%比(1.10±0.07)%,P<0.05],别嘌醇组、麻黄组和黄柏组大鼠血SUA水平显著降低[(46.89±9.90)mmol/L、(42.05±8.23)mmol/L、(51.13±13.86)mmol/L比(77.96±19.40)mmol/L,P<0.01],且麻黄组大鼠血SUA水平与别嘌醇组和黄柏组无差异(P>0.05),麻黄组大鼠尿pH较高[(7.67±0.58)比(6.82±0.40),P<0.05]。结论麻黄能降低高尿酸血症模型大鼠血尿酸水平,可能与调节尿pH有关。

Objective To investigate the relationship between the metabolic effect of Ephedra on serum uric acid(SUA) and urine pH in hyperuricemia(HUA) rats. Methods 30 male SD rats were randomly divided into normal group, model group, allopurinol group, ephedra group and amur cork-tree(Cortex Phellodendri) group with 6 rats in each group. The rat model of HUA was established by intraperitoneal injection of potassium oxalate and hypoxanthine. Then, the rats in Ephedra group were given 20 mL·kg^-1·d^-1 water decoction of ephedra, 20 mL·kg^-1·d^-1 water decoction of Cortex Phellodendri, 20 mL·kg^-1·d^-1 suspension of allopurinol, and the normal group and model group were given equal volume distilled water. Rats were sacrificed after 7 days of regular and quantitative administration. Liver index(LI), kidney index(KI), SUA, serum creatinine(SCr), 24-hour urine volume and uric pH were measured. Results Compared to the normal group, KI of model group and allopurinol group increased [(1.10 ±0.07)% and(1.07±0.04)% vs(0.90±0.05)%, P<0.05, respectively], KI of ephedra group and Cortex Phellodendri group increased significantly [(1.28±0.17)% and(1.18±0.14)% vs(0.90±0.05)%, P<0.01, respectively], level of SUA was obviously higher in model group[(77.96±19.40) vs(40.66±16.77), P<0.01], but no difference in allopurinol group, ephedra group and Cortex Phellodendri group [(46.89 ±9.90),(42.05 ±8.23) and(51.13 ±13.86) vs(40.66±16.77), P>0.05, respectively], urine volume of model rats increased[(29.50±5.25) vs(7.00±2.30), P<0.01],uric pH were higher in allopurinol group and Cortex Phellodendri group[(7.55 ±0.83) and(7.63 ±0.73) vs(6.52 ±0.92), P<0.05, respectively], and uric pH of ephedra group increased significantly[(7.67±0.58) vs(6.52±0.92), P<0.01]. Compared to the model group, KI of ephedra group was slightly higher[(1.28±0.17)% vs(1.10±0.07)%, P<0.05], SUA level of allopurinol group, ephedra group and Cortex Phellodendri group decreased significantly [(46.89±9.90),(42.05±8.23) and(51.13±13.86) vs(77.96±19.40), P<0.01, respectively], but no difference among these groups. The uric pH of ephedra group was higher than that of model group[(7.67±0.58) vs(6.82±0.40), P<0.05].Conclusion Ephedra can reduce the level of serum uric acid in hyperuricemia model rats, which may be related to the regulation of uric pH.