小鼠经腰段鞘内置管的建立方法

Establishment of a novel intrathecal catheterization in mice lumber spinal subarachnoid space

目的介绍改良的小鼠经腰段鞘内置管的方法,旨在为神经学和疼痛学研究提供更安全、方便、高效的小鼠鞘内置管技术。方法 60只雄性昆明小鼠,随机分为改良组和Wu方法组,分别接受经改良和传统的Wu方法行小鼠鞘内置管术,记录两组小鼠导管泄漏率、瘫痪率、死亡率、置管成功率,以及热甩尾潜伏期(TFL)、50%机械缩足阈值(MWT)、导管滑脱率。解剖脊髓观察脊髓损伤情况及置管情况。结果两组小鼠在死亡率、TFL、50%MWT方面比较,差异无统计学意义(P>0.05),改良组与Wu方法组比较,成功率提高,瘫痪率、导管泄漏率、导管滑脱率降低(P<0.05)。结论改良的鞘内置管方法较传统的Wu方法更加安全易行。

Objective To introduce a modified method of catheterization in mice lumbar subarachnoid space with better safety, convenience and efficiency. Methods Male Kunming mice were randomly divided into two groups(n = 30): the modified group and the Wu group. Mice received intrathecal catheterization. Catheter leakage rate, paralysis rate, mortality, and success rate, tail flick frequency, 50% mechanical withdrawal threshold, catheter slip rate, and spinal cord catheterization condition after dissecting spinal cord were recorded. Results There was no statistically significant difference in mortality, tail flick frequency, 50% mechanical withdrawal threshold between the two groups(P > 0.05). The success rate was significantly improved while paralysis rate, the catheter leakage rate and the catheter slip rate were decreased obviously when compared with the Wu group(P < 0.05). Conclusions The novel intrathecal catheterization method is safe and easy to handle.