回阳固元方对人乳头瘤病毒感染细胞凋亡的影响研究

Effect of HuiYangGuYuan prescription on cell apoptosis of human papillomavirus infection

目的探讨回阳固元方对人乳头瘤病毒感染细胞凋亡的影响。方法用不同浓度回阳固元方,经Transwell(转换池)吸收后,与尖锐湿疣患者皮损中提取的角质形成细胞-单细胞悬液作用,通过流式细胞仪测定其细胞周期及细胞凋亡。结果固定中药浓度20μg/ml,随着时间的变化,G1期细胞数量减少,G2期细胞数量逐渐增多,S期细胞数量较正常组减少,角质形成细胞的凋亡率也逐渐增加;固定时间48 h,随着中药浓度的增加,G1期细胞数量降低,G2期细胞数量极少,S期细胞较正常组减少,角质形成细胞的凋亡率呈逐渐增加的趋势(P<0.05)。结论回阳固元方可诱导人乳头瘤病毒感染细胞的凋亡。

Objective To investigate the effect of HuiYangGuYuan Prescription on apoptosis of human papilloma virus infected cells. Methods Keratinocytes extracted from skin lesions of condyloma acuminatum patients was co-incubated with different concentrations of HuiYangGuYuan Prescription in Transwell pool system.Cell suspension was measured by flow cytometry. Cell cycle and apoptosis status was obtained. Results When concentrations of HuiYangGuYuan Prescription was remained on 20 μg/ml, the number of G1 and S cells cell was decreased while G2 cells and apoptosis rate were increased along with increase of incubation time. When cells was co-incubated with HuiYangGuYuan Prescription for 48 hours, the number of G1 cell and S cells was significantly decreased while the apoptosis rate was increased in dose dependent manner(P < 0.05). Conclusion Hui Yang Gu Yuan Prescription may induce human papilloma virus infected cells.