长链非编码RNA MALAT1与细胞凋亡诱导因子在早产儿支气管肺发育不良中表达及意义

Significance of long non-coding RNA MALAT1 and apoptosis induced factor expression in premature infants with bronchopulmonary dysplasia

目的探讨支气管肺发育不良(BPD)早产儿外周静脉血中长链非编码RNAMALAT1及细胞凋亡诱导因子(AIF)表达与BPD的关系。方法采用前瞻性病例对照研究方法,选取2015年1月至2016年12月新生儿科收治的20例BPD早产儿作为BPD组,选取20例同期收治吸氧时间<3天且胎龄<32周的早产儿作为对照组。收集比较两组早产儿的临床资料,以Real-TimePCR方法检测的外周血MALAT1和AIF表达水平。结果与对照组相比,BPD组早产儿外周血中MALAT-1表达上升,AIF表达下降,两组间差异均有统计学意义(P<0.05)。结论长链非编码RNAMALAT1与AIF可能参与BPD发病。

Objective To observe long non-coding RNA MALAT1 and AIF expression in the peripheral venous blood of premature infants with bronchopulmonary dysplasia,and its relationship with BPD.Methods Twenty cases of premature infants with bronchopulmonary dysplasia admitted to the Neonatology Department of Shanghai Children's Hospital from January 2015 to December 2016 were selected as BPD group,and 20 cases of premature infants whose oxygen inhalation time less than 3 days and gestational age less than 32 weeks were selected as control group.Clinical data of 40 cases of premature infants and the residual peripheral blood samples during physical examinations in this hospital were retrospectively analyzed,the expression levels of MALAT1 and AIF in serum were measured by real-time PCR.The data of two groups were compared by t test.Results(1)Compared with the control group,the expression of MALAT1 in peripheral blood of premature infants in BPD group were significantly increased[0.2734±0.0673 vs 0.3755±0.0819,P<0.05].(2)Compared with the control group,the expression of AIF in peripheral blood of premature infants in BPD group were significantly decreased[0.0068±0.00196 vs 0.0045±0.00187,P<0.05].Conclusion The premature infants with BPD have abnormal expression of long non-coding RNA MALAT1 and AIF in the blood.Such abnormal changes may be involved in the pathogenesis of BPD.