基于肝脏代谢组分析研究低聚硒化氨基多糖对黑鲷的免疫调节作用

Immunomodulatory effect of low relative molecular mass seleno-aminopolysaccharide in Acanthopagrus schlegelii by hepatic metabolomics analysis

采用基于液相色谱-飞行时间质谱联用(LC-TOF-MS)技术的代谢组学方法,分析黑鲷肝脏内源性代谢物的变化,研究硒化氨基多糖增强黑鲷的免疫调节机制。采用XCMS plus 软件非靶向分析质谱采集数据,筛选潜在生物标志物,并通过MetaboAnalyst3.0网站分析相关代谢通路。结果表明,饲喂硒化氨基多糖组中的代谢物明显区分于空白组,发现并鉴定了32个有差异的生物标志物。代谢通路分析结果表明,硒化氨基多糖可通过氨基酰基-转运脱氧核糖核苷酸(tRNA)生物合成、氨基酸代谢、核苷酸代谢、氮代谢等代谢通路增强黑鲷自身的免疫机能。该研究为阐明硒化氨基多糖的免疫增强机制提供了科学依据。

To investigate the immunomodulatory mechanism of low relative molecular mass seleno -aminopolysaccharide, a metabolomics method based on liquid chromatography-time-of-flight mass spectrometry (LC-TOF-MS) was used to analyze the endogenous metabolites changes in the liver of Acanthopagrus schlegelii . The potential biomarkers were screened using non-targeted mass spectrometry with the XCMS plus software, and the related metabolic pathways were analyzed using MetaboAnalyst3.0 website. The results showed that the liver metabolites in the low relative molecular mass seleno-aminopolysaccharide-fed group were significantly different from those in the blank group. Also, 32 biomarkers were identified. Additionally, low relative molecular mass seleno-aminopolysaccharide could enhance the immune function of Acathopagrus schlegelii via amino acid, nucleotide, and nitrogen metabolism, aminoacyl- transfer ribonucleic acid (tRNA) biosynthesis, and other metabolic pathways. This study therefore provides a scientific basis for elucidating the immunoenhancement mechanism of low relative molecular mass seleno-aminopolysaccharide.